Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4

Retroviral expression vector of human Kruppel like factor 4 (KLF4).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19004_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4 5') Sequence file: RDB19004_B1Eca.seq
>D07716A1_B1Ec_4_MMLV-pol_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNANNNGG NNTCGCANCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA   121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG   181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT   241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA   301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC   361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG   421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG   481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG   541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC   601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG   661 GCGTGGCGCC GGGCGGCACG GGCGGAGGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC   721 CGACGGCTCC CTTCAACCTG GCGGANATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG   781 CCGAGCTCCT GCGGCCAGAN TTGGACCCGG TGTACATTCC GCCGCAGCAG CCGCAGCCGC   841 CAGGNGGCGG GCTGATGGGC AANTNCGTGC TNAANGNGTC NCTGANCGCC CCTGGCAGCG   901 ANNNNNNNAG CCCGNNNNTC ATCAGCGNCA GCNANGNCAN NCCNNANNGC NGCCNNCCGG 961 NGGTGGNGGC GNCCNNCNNN NGNNGGNNNN NNNCGCNNGN NNCCNNANNN NNNNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4 3') Sequence file: RDB19004_B1Ecb.seq
>D07716A1_B1Ec_4_pMXs-3LTRup_R2_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTTNNNCGN CNNNNNCTGT GCTGGCGGCC GCTTAAAAAT    61 GCCTCTTCAT GTGTAAGGCG AGGTGGTCCG ACCTGGAAAA TGCTCGGTCG CATTTTTGGC   121 ACTGGAACGG GCGGTGCCCC GTGTGTTTAC GGTAGTGCCT GGTCAGTTCA TCTGAGCGGG   181 CGAATTTCCA TCCACAGCCG TCCCAGTCAC AGTGGTAAGG TTTCTCACCT GTGTGGGTTC   241 GCAGGTGTGC CTTGAGATGG GAACTCTTTG TGTAGGTTTT GCCGCAGCCC GCGTAATCAC   301 AAGTGTGGGT GGCGGTCCTT TTCCGGGGCC ACGATCGTCT TCCCCTCTTT GGCTTGGGCT   361 CCTCTGGCAT GCAGGAACCG GGTGGCATGA GCTCTTGGTA ATGGAGCGGC GGGACTTGCG   421 GCTGCATCTG ATCGGGCAGG AAGGATGGGT AATTGGGCCC CGGGTGGGGA TGGAAGCCGG   481 GAGGAAGCGG CAGGGCAGGG TGACAGTCCC TGCTGCTCAG CACTTCCTCA AGACCCAGGG   541 TCGGGGTAGT CCTGCTGGGG AGCTGCCGCC CCAGGGGGAA GTCGTGTGCA GCCGGCCGGT   601 GGCCATTGCT GAGAGGGGGT CCAGCGCCCA AGTGGGTGCA CGAAGAGACC GCCTCCTGCT   661 TGATCTTGGG GCACGTGCGC GGCGGCCCGC CGTTGTAGGG CGCCACCACC ACCGGGTGGC   721 TGCCGTCAGG GCTGCCTTTG CTGACGCTGA TGACCGACGG GCTGCCGTAC TCGCTGCCAG   781 GGGCGCTCAG CGACGCCTTC AGCACGAACT TGCCCATCAG CCCGCCACCT GGCGGCTGCG   841 GCTGCTGCGG CGGANTGTAC ACCGGGNCNN ATTCTGGCCG CAGGANCTCG GCCACNAANN   901 CGCCCNANGG GCTCACGTCN TTGANGTCCG CCNGGNTNNA NGGNNNCCNN NNNANGGGGA   961 NCNNNNTCCC NGCCNTNNNN NANNNNTNCG NCCGNNNNNN CCGGNNCCNC NCCNNGNNNN 1021 NNNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19004_B1Ecc.seq
>D07716A1_B1Ec_4_gag-N_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNTNNTTTT NNGANNAATA CNGAAACACA GTCAGACAGA    61 GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC GCGGCTTCGG TCCCAAACCG   121 AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT CTCGTCTCCT ACCAGAACCA   181 CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA ACGATCGGGA TTTTTGGACT   241 CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC GTCTCCCAGG GTTGCGGCCG   301 GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA TACAGAGCTA GTTAGCTAAC   361 TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA GACAATCGGA CAGACACAGA   421 TAAGTTGCTG GCCAGCTTAC CTCCCGGTGG TGGGTCGGTG GTCCCTGGGC AGGGGTCTCC   481 CGATCCCGGA CGAGCCCCCA AATGAAAGAC CCCCGCTGAC GGGTAGTCAA TCACTCAGAG   541 GAGACCCTCC CAAGGAACAG CGAGACCACA AGTCGGATGC AACTGCAAGA GGGTTTATTG   601 GATACACGGG TACCCGGGCG ACTCAGTCAA TCGGAGGACT GGCGCGCCGA GTGAGGGGTT   661 GTGGGCTCTT TTATTGAGCT CGGGGAGCAG AAGCGCGCGA ACAGAAGCGA GAAGCGAACT   721 GATTGGTTAG TTCAAATAAG GCACAGGGTC ATTTCANGTC CTTGGGGCAC CCTGGAAACA   781 TCTGATGGTT CTCTAGAAAC TGCTGANGGC TGGACCGCAT CTGGGGANNA TCTGTTCTTG   841 GNCCTGANCC NGGGCAGGAA CTGCTTANCA CAGATATCCT GTTTGGNCNN TATTCNGCTG   901 NNCCNTCTGN NCTNNNNCCT NANCCNGGNN ANGAACNGNT NANNNCAGNN ATNCNGNTNG 961 GNCNNTATTC NGCTNNNNNA TNNNNNNNNN ANNNTNNNNN NNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19004_B1Ecd.seq
>D07716A1_B1Ec_4_lacIpro-B_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNCGNNTNNG TGAGCGAGGA AGCGGAAGAN CGCCCAATAC    61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC   481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT   541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA   601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT   661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT   721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA   781 AATAAAATCT TTTATTTTAT CGTCGACCAC TGTGCTGGCG GCCGCTNNNN NTGCCTCTTC   841 ATGTGTAANG CGAGGTGGTC CGACCTNGAA AANGCTCGGT CGCATTTTTG GCACTGGAAC   901 GGGCGGNGCC CCNNGTGTTT ANNGNANTGN CTGGNCAGTT CATCTGANCG GNNGANNTTC   961 CNTCCNNAGC CNNCCNNNNN NCAGNGGTNN NNNTNCNCNN NTGNGTGGNN NCNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

GNP_filter3_RDBDEP_html_230702.pl