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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA5-FRT-TO-EGFP-Mo Climp63

Expression vector of mouse Climp63/Ckap4 tagged with EGFP.

Catalog number RDB19222
Resource name pcDNA5-FRT-TO-EGFP-Mo Climp63
Clone info. Expression vector of mouse cytoskeleton-associated protein 4 (Climp63/Ckap4) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA5-FRT-TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell)
Gene/insert name mouse Ckap4 cDNA
Depositor|Developer Nukina, Nobuyuki | Yamanaka, Tomoyuki |
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External Database
mouse Ckap4

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020)。
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          RDB19222 pcDNA5-FRT-TO-EGFP-Mo Climp63 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA5-FRT-TO-EGFP-Mo Climp63 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19222).

          Reference section:

          Yamanaka, T., Nishiyama, R., Shimogori, T., Nukina, N., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919.

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19222_B1Gjp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, tetO2, EGFP 5'
          Sequence file: RDB19222_B1Gja.seq check
          1
          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3', insert 5'
          Sequence file: RDB19222_B1Gjb.seq check
          1
          Primer: BGH_rev2 (Pr0606)
          Region: insert 3'
          Sequence file: RDB19222_B1Gjc.seq check
          1
          Primer: BGHpA_F (Pr0373)
          Region: bGHpolyA, FRT, HygR
          Sequence file: RDB19222_B1Gjd.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yamanaka, T., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl