Resource data sheet
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pcDNA5-FRT-TO-EGFP-Mo Climp63 (#RDB19222)

Expression vector of mouse Climp63/Ckap4 tagged with EGFP.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Yamanaka, T., Sci. Rep. 10 (1): 7610 (2020). PMID 32376919. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse cytoskeleton-associated protein 4 (Climp63/Ckap4) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence microscopy.
Vector backbone pcDNA5-FRT-TO (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin (E. coli), Hygromycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name mouse Ckap4 cDNA
Depositor|Developer Nukina, Nobuyuki | Yamanaka, Tomoyuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Yamanaka, T. et al., Sci. Rep., 10 (1): 7610, 2020)。

Catalog # Resource name Shipping form Fee
RDB19222 pcDNA5-FRT-TO-EGFP-Mo Climp63 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA5-FRT-TO-EGFP-Mo Climp63 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19222).

Reference section:

Yamanaka, T., Nishiyama, R., Shimogori, T., Nukina, N., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Yamanaka, T., Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation. Sci. Rep. 10 (1): 7610 (2020). PMID 32376919. [PubMed] [Article] [RRC of NBRP]