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pAID1.2-CMV-NmScarlet-mAID (#RDB19226)

Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by CMV promoter for the super-sensitive AID system.

Clone info. Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously under control of the CMV promoter for the super-sensitive auxin-inducible degron (AID) system. See Fig.1 of Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), G418 (mammalian cell)
Gene/insert name oriza sativa OsTIR1 cDNA
Depositor|Developer Fukagawa, Tatsuo |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19226 pAID1.2-CMV-NmScarlet-mAID DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAID1.2-CMV-NmScarlet-mAID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19226).

Reference section:

Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19226_B1H2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, OsTIR1 5'
Sequence file: RDB19226_B1H2a.seq check
>D08293A1_B1H2_3_CMV_Forward_A01_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNNNNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTGCCACCAT GACATACTTT
  121 CCTGAAGAGG TCGTCGAACA CATTTTTAGC TTCCTGCCTG CACAGAGAGA TAGAAACACA
  181 GTGAGCCTGG TCTGCAAAGT GTGGTACGAG ATCGAACGCC TGAGCCGGAG AGGAGTGTTC
  241 GTCGGCAACT GCTATGCTGT GAGAGCAGGC AGGGTCGCCG CTAGGTTTCC AAATGTGCGC
  301 GCACTGACCG TCAAGGGGAA ACCCCACTTC GCCGACTTTA ACCTGGTGCC CCCTGATTGG
  361 GGAGGATACG CCGGCCCTTG GATCGAGGCA GCCGCTCGCG GCTGTCATGG ACTGGAGGAA
  421 CTGCGCATGA AGCGAATGGT GGTCTCTGAC GAAAGTCTGG AGCTGCTGGC TCGGAGCTTC
  481 CCTAGGTTTC GCGCACTGGT GCTGATTTCT TGCGAAGGCT TCAGCACCGA TGGACTGGCA
  541 GCCGTGGCCT CCCACTGTAA GCTGCTGCGG GAGCTGGACC TCCAGGAGAA TGAAGTGGAG
  601 GATAGAGGCC CCAGATGGCT GTCTTGCTTC CCAGACTCAT GTACCAGCCT GGTGTCCCTG
  661 AACTTTGCCT GCATCAAAGG CGAAGTGAAT GCTGGGTCCC TGGAGCGGCT GGTCTCAAGA
  721 AGCCCCAACC TGAGGTCTCT GCGGCTGANC CGGANCGTGA GCGTGGACAC TCTGGCTAAG
  781 ANTCTGCTGA NAACCCCTAA CCTGGANGAN CTGGGAACCG GCAATCTGAC AGANGANTTC
  841 CNGACAGANT CCTACTTTAA ACTGACTTCT GNCCTGGANA ANTGTAAAAT GNTGANGANT
  901 CTGTCNNNTT CTGGGATGCT TNACCCNNNN GNNNGANCTT TATCNNCCCN NNGNGNGCNN
961 AGCTGANNNN NNNGNNNNNN ANNNNTGCAN NNNNCNNNNN NNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert (mini AID, mScarlet 3')
Sequence file: RDB19226_B1H2b.seq check
>D08293A1_B1H2_3_SV40polyA_R2_D01_10_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNN NNNGTTANNN ACNACAATTG GATATCGGCA CCCGCTCCNG
   61 CGCCTGCACC AGCTCCTTTA TACATCCTCA AATCGATTTT CCTCAAGTAC GGTGCTCCGT
  121 CCATTGATAC CTTCACGAAC GCCGCCGCCT CCGGGCCACC GCTTGATTTT TGGCAGGAAA
  181 CCATCACGTT CTTCCGGTAT GATCTCACCG GTGGCCATCC CACAACTTGT GCCTTGGCCG
  241 GAGGTTTGGC TGGATCTTTA GGACAAGCAC TCTTCTCCTT CTTGTACAGC TCGTCCATGC
  301 CGCCGGTGGA GTGGCGGCCC TCGGAGCGTT CGTACTGTTC CACCACGGTG TAGTCCTCGT
  361 TGTGGGAGGT GATGTCCAAC TTGCGGTCGA CGTTGTAGGC GCCGGGCATC TGCACGGGCT
  421 TCTTGGCCTT GTAGGTGGTC TTGAAGTCCG CCAGGTAGCG GCCGCCGTCC TTCAGGCGCA
  481 GGGCCATCTT AATGTCGCCC TTCAGCACGC CGTCCTCGGG GTACAACCGC TCGGTGGACG
  541 CTTCCCAGCC CATTGTCTTC TTCTGCATTA CGGGGCCGTC AGGAGGGAAG TTGGTGCCGC
  601 GGAGCTTCAC CTTGTAGATC AGGGTGCCGT CCTCCAGGGA GGTGTCCTGG GTCACGGTCA
  661 CGGCGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAANGANT
  721 GCTTATAGTA GTCGGGGANG TCGGCGGGGT GCTTGATGAA GGCCCTGGAG CCGTACATGA
  781 ACTGANGGGA NAGGATGTCN CAGGAGAANG GCANGGGGCC ACCCTTGGTC ACCTTCAGCT
  841 TGGCGGTCTG GGTGNCCTCN NAGGGGCGGC CCTCGCCCTC GCCCNCGATC NCNAANTCNN
  901 GGCCGTNCAT GGANCCCNNC NTGTGCACCT TGNNCCGCAT GAACTCCNNG ATCACTGNNN
961 CNCCCTTGNN CANCANNNNA TCGCACATNN NNNNNNNN
//
Primer: IRES-R (Pr0037)
Region: insert (bsr, T2A, OsTIR1 3')
Sequence file: RDB19226_B1H2c.seq check
>D08293A1_B1H2_3_IRES-R_A09_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNGNNNNNN NNNNNANGGG
   61 CGGATCCCGG GGNTCTCTTG AAATGATCGT TCCACCTCAA AGGGGCTTCA TGATGGTCCC
  121 CATAATTTTT GGCAGAGGGA AAAAGATCNN NNNTTTCGGG TATATTTGAG TGGAATGAGT
  181 TCTTCAATCG TAGTTTTGAC TAACTTGCCA TTCATTTCTA TTAACACAAA ACAATCTGGT
  241 GCATAGTCTG AAATCAACTC CCTACACATA CCACAAGGAC TTACCACTCG AATACTTCTA
  301 TCTACTTCGT CAGAATAAGG GTGTCTAACA GCTACAATCG TGTCAAAATC CTTTTGTCCA
  361 TTCGAAACTG CACTACCAAT CGCAATGGCT TCTGCACAaA CaGTTACTCG TCCTATATAC
  421 GCTTCAATAT GTACTGCCGA AATGATTTCT CCTGTTTTCG TACGAATTGC CGCTCCCACA
  481 TGATGTTTAT TATCCTCATA AAGCATTGTA ATCTTCTCTG TCGCTACTTC TACTAATTCT
  541 AGATCTTGTT GAGAAATGTT AAATGTTTTC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG
  601 CAGGTCAGCA GGCTGCCGCG GCCCTCCAGA ATCTTCACAA AGTTGGGAGC ATCNTCTCTT
  661 GCCCCTGCGG TGGTCCGAAT ACACATACAG TTTCTCCNNC TTTGGCAGGT CNCCATGATT
  721 TTCCTCNNNT TCGTTAGACC NNNNCCGCTC ATTAATGANT TCCACGTTCA NNATAGGCAT
  781 TTTACTAGCC AGGANCNTGA CAGCCCTTCA NAGTCACATT GCAACTANAC ATCCANNNGC
  841 TCCTCATNGT CTNCNNANNT GGCNNANNNT TCCCNNNNNA NCGGNCNNTC CCNNAAANNG
  901 NNTGNNNCCG NANTTNCNNG TTTTNCGCAN ATNCTTACNC CCCNTTCATC NNGNNNNTNN
  961 NGNCCTTANN NNNANNNNNN NNCNAATGCA ANNNTNANCA TCTCCNNNNN GNNTTANNAN
1021 ANTTGCCNAA NGNNNNTGNN NNNNTTTNNN NNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES2, mScarlet 5'
Sequence file: RDB19226_B1H2d.seq check
>D08293A1_B1H2_3_IRES-F_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN NNNNNANGTT NNNNNNNCGT CTAGGCCCCC CGAACCNCGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG TGCGATAACA
  121 TGGTGAGCAA GGGCGAGGCA GTGATCAAGG AGTTCATGCG GTTCAAGGTG CACATGGAGG
  181 GCTCCATGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC CCCTACGAGG
  241 GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC TCCTGGGACA
  301 TCCTGTCCCC TCAGTTCATG TACGGCTCCA GGGCCTTCAT CAAGCACCCC GCCGACATCC
  361 CCGACTACTA TAAGCAGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG ATGAACTTCG
  421 AGGACGGCGG CGCCGTGACC GTGACCCAGG ACACCTCCCT GGAGGACGGC ACCCTGATCT
  481 ACAAGGTGAA GCTCCGCGGC ACCAACTTCC CTCCTGACGG CCCCGTAATG CAGAAGAAGA
  541 CAATGGGCTG GGAAGCGTCC ACCGAGCGGT TGTACCCCGA GGACGGCGTG CTGAAGGGCG
  601 ACATTAAGAT GGCCCTGCGC CTGAAGGACG GCGGCCGCTA CCTGGCGGAC TTCAAGACCA
  661 CCTACAAGGC CAAGAAGCCC GTGCAGATGC CCGGCGCCTA CAACGTCGAC CGCAAGTTGG
  721 ACATCACCTC CCACAACGAG GACTACACCG TGGTGGAACA GTACGAACGC TCCGANGGCC
  781 GCCACTCCAC CGGCGGCNTG GACGANCTGT ACNAGAANGA NAAGANTGCT TGTCCTAAAG
  841 ATCCAGCCNA ACCTCCGGCC AANGNACAAG TNGTGGGATG GNCNCCGGTG ANANCATACC
  901 GGAANANNGN GATGGTTNCN NGNNNAAAAT CAAGCGGNNG NCCNGANGNN NNGGNNTTCN
961 NGNNNNNNTC NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB19226_B1H2e.seq check
>D08293A1_B1H2_3_SV40pro_ori_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTCG GCCTCTGNNC TATTCCNGAA GTAGTGAGGA
   61 GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA GATCGATCAA GAGACAGGAT GAGGATCGTT
  121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  301 ACTGCAAGAC GAGGNNNCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
  781 NGACATAGCG TTGGCTACCC GTGANATTGC TGAAGAGCTT GGCGGCNAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACNGGN ATCGCCGCTC CCGATTCGCA NCGCATCGCC TTCTATCGNN
  901 NNNTTGACNA NTNCTTCTGA NNNGNANTCT GGGGNNNGAA ATGANCGANC NANCNACNNC
961 NNACCNNNCN TCACNANNNT TNNATTNCNN CNGNCNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]