Please review the QC test results indicated by icon below as well as clone information before placing your order.

pAIDFA-CMV-NmScarlet-mAID

Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 mutant (F74A) simultaneously by CMV promoter for the super-sensitive AID system.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19228_B1H2p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(OsTIR1F74A 5') Sequence file: RDB19228_B1H2a.seq
>D08293A3_B1H2_2_CMV_Forward_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNCN TNNNNNNNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTGCCACC ATGACATACT   121 TTCCTGAAGA GGTCGTCGAA CACATTTTTA GCTTCCTGCC TGCACAGAGA GATAGAAACA   181 CAGTGAGCCT GGTCTGCAAA GTGTGGTACG AGATCGAACG CCTGAGCCGG AGAGGAGTGT   241 TCGTCGGCAA CTGCTATGCT GTGAGAGCAG GCAGGGTCGC CGCTAGGTTT CCAAATGTGC   301 GCGCACTGAC CGTCAAGGGG AAACCCCACG CCGCCGACTT TAACCTGGTG CCCCCTGATT   361 GGGGAGGATA CGCCGGCCCT TGGATCGAGG CAGCCGCTCG CGGCTGTCAT GGACTGGAGG   421 AACTGCGCAT GAAGCGAATG GTGGTCTCTG ACGAAAGTCT GGAGCTGCTG GCTCGGAGCT   481 TCCCTAGGTT TCGCGCACTG GTGCTGATTT CTTGCGAAGG CTTCAGCACC GATGGACTGG   541 CAGCCGTGGC CTCCCACTGT AAGCTGCTGC GGGAGCTGGA CCTCCAGGAG AATGAAGTGG   601 AGGATAGAGG CCCCAGATGG CTGTCTTGCT TCCCAGACTC ATGTACCAGC CTGGTGTCCC   661 TGAACTTTGC CTGCATCAAA GGCGAAGTGA ATGCTGGGTC CCTGGAGCGG CTGGTCTCAA   721 GAAGCCCCAA CCTGANGTCT CTGCGGCTGA NCCGGANCGT GAGCGTGGAC ACTCTGGCTA   781 AGATTCTGCT GANAANCCCT ANCCTGGAGG ATCTGGGAAC CGGCAATCTG ACAGACGATT   841 TCCAGANAGA NTCCTACTTT AAACTGACTT CTGCCCTGGA NAANTGTAAA ATGCTGANGA   901 NTCTGTCNNN NNNTGGGANG CTTCNCCCGN GNGCCTGANC TTTATCTACC CNCNNNGTNN 961 NCAGCTNANN NGNCTNANNN NNANCNNTGN NCNNNCCNNN NNNGCNNNNN NNNNN //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3'(mini-AID,mScarlet 3') Sequence file: RDB19228_B1H2b.seq
>D08293A3_B1H2_2_SV40polyA_R2_C09_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNNNNACN NNNNTTGGNT ATCGGCACCC    61 GCTCCNGCGC CTGCACCAGC TCCTTTATAC ATCCTCAAAT CGATTTTCCT CAAGTACGGT   121 GCTCCGTCCA TTGATACCTT CACGAACGCC GCCGCCTCCG GGCCACCGCT TGATTTTTGG   181 CAGGAAACCA TCACGTTCTT CCGGTATGAT CTCACCGGTG GCCATCCCAC AACTTGTGCC   241 TTGGCCGGAG GTTTGGCTGG ATCTTTAGGA CAAGCACTCT TCTCCTTCTT GTACAGCTCG   301 TCCATGCCGC CGGTGGAGTG GCGGCCCTCG GAGCGTTCGT ACTGTTCCAC CACGGTGTAG   361 TCCTCGTTGT GGGAGGTGAT GTCCAACTTG CGGTCGACGT TGTAGGCGCC GGGCATCTGC   421 ACGGGCTTCT TGGCCTTGTA GGTGGTCTTG AAGTCCGCCA GGTAGCGGCC GCCGTCCTTC   481 AGGCGCAGGG CCATCTTAAT GTCGCCCTTC AGCACGCCGT CCTCGGGGTA CAACCGCTCG   541 GTGGACGCTT CCCAGCCCAT TGTCTTCTTC TGCATTACGG GGCCGTCAGG AGGGAAGTTG   601 GTGCCGCGGA GCTTCACCTT GTAGATCAGG GTGCCGTCCT CCAGGGAGGT GTCCTGGGTC   661 ACGGTCACGG CGCCGCCGTC CTCGAAGTTC ATCACGCGCT CCCACTTGAA GCCCTCGGGG   721 NAAGGACTGC TTATANNAGT CGGGGATGTC GGCGGGGTGC TTGGTGAAGG NCCTGGAGCC   781 GTACATGAAC TGANGGGANA GGATGTCCCA NGANAAGGGC AGGGGGCCAC CCTTGGTCAN   841 CTTCAGCTNG GCGGNNTGGG NGCCCTCGTA NNNGCGGCCC TCGCCCTCNC CCNCNATCTC   901 NAACTCGNGN CCGTNCNNGN NNCCCNCCAT GTGCNNCTNG NAANCNNATG ANNTCCTTGA   961 TCACTGNCNN CGNCCNNTGN NNANNANGNN NNNNGNACAT NGNNNNNNGG NCATNNNNNC 1021 NTCNNGNTTT TTCAAANGAA ANNNNNNNNC CCNNNNNNNN NN //
Primer: IRES-R (Pr0037) Region: insert mid(IRES2,bsr,T2A,OsTIR1F74A 3') Sequence file: RDB19228_B1H2c.seq
>D08293A3_B1H2_2_IRES-R_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNNNNNNGGG GGGGGNNGGA NAGGGGCGGA    61 TCCCGGGGAT CTCTTGAATG ATCGTTCCAC CTCAAGGGGC TTCATGATGT CCCCATAATT   121 TTTGGCAGAG GGAAAAAGAT CCTTAATTTC GGGTATATTT GAGTGGAATG AGTTCTTCAA   181 TCGTAGTTTT GACTAACTTG CCATTCATTT CTATTAACAC AAAACAATCT GGTGCATAGT   241 CTGAAATCAA CTCCCTACAC ATACCACAAG GACTTACCAC TCGAATACTT CTATCTACTT   301 CGTCAGAATA AGGGTGTCTA ACAGCTACAA TCGTGTCAAA ATCCTTTTGT CCATTCGAAA   361 CTGCACTACC AATCGCAATG GCTTCTGCAC AAACAGTTAC TCGTCCTATA TACGCTTCAA   421 TATGTACTGC CGAAATGATT TCTCCTGTTT TCGTACGAAT TGCCGCTCCC ACATGATGTT   481 TATTATCCTC ATAAAGCATT GTAATCTTCT CTGTCGCTAC TTCTACTAAT TCTAGATCTT   541 GTTGAGAAAT GTTAAATGTT TTCATGGGGC CGGGGTTCTC CTCCACGTCG CCGCAGGTCA   601 GCAGGCTGCC GCGGCCCTCC AGAATCTTCA CAAAGTTGGG AGCATCATCT CTTGCCCCTG   661 CGGTGGTCCG ATACACATAC AGTTTCTCCA CCTTTGGCAG GTCGCCATGA TTTTCCTCCA   721 TTTCGTTAGA CCCGTCCCGC TCATTAATGA CTTCCACGTT CAGCATANGC ATTTTACTAG   781 CCAGGACCTG ACAGCCCTTC AGAGTCACAT TGCAACTAGA CATCCACNGG CTCCTCATTG   841 TCTCGTATCT GGCAAAGTTT CCCAGCANAN CGGCATCCCC GAAAGGGNTG TCCCGGNATT   901 TCCAGNTTTC NNANANNCTT ACNCCCGTTC ATCACGTGCA TCATGCCCTT ATCGGANTCT   961 CCNNNNANTG NNATGNTCNN NNNCTCNAGC TGNNTANCNT ACNTGNCNAN NTANNNGNNN 1021 NNTTNNNNTG NCANNNNNNN NNN //
Primer: IRES-F (Pr0036) Region: insert mid(IRES2,mScarlet 5') Sequence file: RDB19228_B1H2d.seq
>D08293A3_B1H2_2_IRES-F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNTNNGT CNAGGNNNNN NANCGTCTAG GCCCCCCGAA CCNCGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGTGCG ATAACATGGT   121 GAGCAAGGGC GAGGCAGTGA TCAAGGAGTT CATGCGGTTC AAGGTGCACA TGGAGGGCTC   181 CATGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC   241 CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCTCCT GGGACATCCT   301 GTCCCCTCAG TTCATGTACG GCTCCAGGGC CTTCACCAAG CACCCCGCCG ACATCCCCGA   361 CTACTATAAG CAGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA   421 CGGCGGCGCC GTGACCGTGA CCCAGGACAC CTCCCTGGAG GACGGCACCC TGATCTACAA   481 GGTGAAGCTC CGCGGCACCA ACTTCCCTCC TGACGGCCCC GTAATGCAGA AGAAGACAAT   541 GGGCTGGGAA GCGTCCACCG AGCGGTTGTA CCCCGAGGAC GGCGTGCTGA AGGGCGACAT   601 TAAGATGGCC CTGCGCCTGA AGGACGGCGG CCGCTACCTG GCGGACTTCA AGACCACCTA   661 CAAGGCCAAG AAGCCCGTGC AGATGCCCGG CGCCTACAAC GTCGACCGCA AGTTGGACAT   721 CACCTCCCAC AACGAGGACT ACACCGTGGT GGAACAGTAC GAACGCTCCG AGGGCCGCCA   781 CTCCACCGGC GGCATGGACG ANCTGTACAA GAANGANAAG AGTGCTTGTC CTAAAGATCC   841 AGCCAAACCT CCGGNCNAGG CACAAGTTGT GGGATGGNCA CCGGNGANAT CATACCGGAA   901 GAANGTGATG GTTTCCNGNN NAAAATCAAG CGGNGGNCNN GANGNNGNGN NNTTCGTGNA   961 NGNTATCAAN GGNNGGNNCA NNNNACTTNN NNAAAATNGN NTTNNNNNNG NNNNANNGNN 1021 NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB19228_B1H2e.seq
>D08293A3_B1H2_2_SV40pro_ori_F_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCGCCTCGNC CTCTGNNNNN TTCCNGAAGT AGTGAGGAGG    61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC   121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT   181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT   241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC   301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG   361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC   421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA   481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC   541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG   601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG   661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA   721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG   781 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT   841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCANCG CATCGCCTTC TATCGCCTTC   901 TTGACGANTT CTTCTGANCG GGNNTCTGGG GGTNCGAAAT GACCGANCNN NCGACGCCNN 961 ACCTGNNNTC NCNANNNTTC NANNNNNNNN NN //
Primer: Sequence file: RDB19228_B1H2f.seq
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Primer: Sequence file: RDB19228_B1H2g.seq
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Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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