Resource data sheet
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pF25AICET7_LYN (#RDB19300)

Protein expresion vector of human LYN proto-oncogene for Promega TnT insect cell cell-free protein-synthesis system.

Alternative name pF25AICET7_LYN
Clone info. Protein expresion vector of human LYN proto-oncogene, Src family tyrosine kinase (LYN) for Promega TnT insect cell cell-free protein-synthesis system. See Fig.3A of Kinoshita-Kikuta, E. et al. Sci. Rep. 10 (1): 16273, 2020.
Comment Expression was confirmed by the depositor with SDS-PAGE, immunoblotting.
Vector backbone pF25A ICE T7 Flexi vector (plasmid)
Size of vector backbone 3.9 kb
Selectable markers Ampicillin
Gene/insert name human LYN cDNA
Depositor|Developer Kinoshita, Emiko |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19300 pF25AICET7_LYN Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pF25AICET7_LYN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19300).

Reference section:

Kinoshita-Kikuta, E., Utsumi, T., Miyazaki, A., Tokumoto, C., Doi, K., Harada, H., Kinoshita, E., Koike, T., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]