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pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP

Targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene.

Catalog number RDB19322
Resource name pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP
Clone info. Clec4f targeting construct ot insert IRES-hDTR-P2A-EGFP into 3'UTR region of mouse Clec4f gene. By using pX330-syn-crRNA-TS-sgRNA (RDB19319), the targeting cassette can be excised in vivo. See Fig.S8A and S8B of Ishibashi, R. et al. Sci. Rep. 10 (1): 14120, 2020.
Vector backbone pBluescriptII SK(+) (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Clec4f genomic human HBEGF
Depositor|Developer Ishibashi, Riki |
Other clones in our bank
External Database
mouse Clec4f human HBEGF
        Reference sequence
          
         
        Sequence (full) RDB19322hts01.seqcheck assembled from experimentally sequenced data
        Test sheet RDB19322_B1Jep1-1.pdf 

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, R. et al., Sci. Rep. 10 (1): 14120, 2020)。
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        RDB19322 pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP DNA solution

        How to cite this biological resource

        Materials & Methods section:

        The pCriMGET_v1-Clec4f-IRES-hDTR-2A-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19322).

        Reference section:

        Ishibashi, R., Abe, K., Ido, N., Kitano, S., Miyachi, H., Toyoshima, F., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)

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        References

        Original, user report and related articles

        original Ishibashi, R., Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence. Sci. Rep. 10 (1): 14120 (2020). PMID 32839482. [link to RRC of NBRP]

        2023.12.26

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