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pEGFPC3_EGFP-hZFAT-HA (ZF-C delta-2)

Expression vector of human ZFAT mutant (C-terminal deletion of 742-1243 a.a) tagged with EGFP and HA.

Catalog number RDB19339
Resource name pEGFPC3_EGFP-hZFAT-HA (ZF-C delta-2)
Clone info. Expression vector of human zinc finger and AT-hook domain containing (ZFAT) mutant (C-terminal deletion of 742-1243 a.a) tagged with EGFP at N-terminus and HA at C-terminus. See Fig.S10 of Ishikura, S. et al. Nucleic Acids Res. 48 (19): 10848-10866, 2020.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pEGFP-C3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human ZFAT cDNA
Depositor|Developer Shirasawa, Senji | Ishikura, Shuhei |
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External Database
human ZFAT

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          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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          RDB19339 pEGFPC3_EGFP-hZFAT-HA (ZF-C delta-2) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

          2023.04.06

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