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pEGFPC3_EGFP-hBRD4

Expression vector of human BRD4 tagged with EGFP.

Catalog number RDB19341
Resource name pEGFPC3_EGFP-hBRD4
Clone info. Expression vector of human bromodomain containing 4 (BRD4) tagged with EGFP at N-terminus.
Comment Expression was confirmed by the depositor with immunofluorescence.
Vector backbone pEGFP-C3 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human BRD4 cDNA
Depositor|Developer Shirasawa, Senji | Ishikura, Shuhei |
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External Database
human BRD4

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR are requested. (Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020) For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること(Ishikura, S. et al., Nucleic Acids Res. 48 (19): 10848-10866, 2020)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行うこと。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19341 pEGFPC3_EGFP-hBRD4 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Original reference

          original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Ishikura, S., ZFAT binds to centromeres to control noncoding RNA transcription through the KAT2B-H4K8ac-BRD4 axis. Nucleic Acids Res. 48 (19): 10848-10866 (2020). PMID 32997115.

          2023.04.06

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