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pCS2+MT-MS2HB-APOBEC1

Expression vector of the catalytic domain of human APOBEC1 tagged with MS2HB at N-terminus for artificial enzymatic RNA editing system.

Catalog number RDB19360
Resource name pCS2+MT-MS2HB-APOBEC1
Clone info. Expression vector of the catalytic domain of human apolipoprotein B mRNA editing enzyme catalytic subunit 1 (APOBEC1) tagged with MS2HB at N-terminus for artificial enzymatic RNA editing system. Please refer to Bhakta, S. et al. Sci. Rep. 10 (1): 17304, 2020. MS2HB, bacteriophage MS2 coat protein dimer fused to a tag composed of two hexahistidine tags, a TEV cleavage site, and a signal sequence for in vivo biotinylation-tag (HTBH-tag). No myc-tag.
Comment Expression was confirmed by the depositor with microscopy.
Vector backbone pCS2 + MT
(plasmid)
Size of vector backbone 4.4 kb
Selectable markers Ampicillin
Gene/insert name Bacteriophage MS2 coat protein cDNA human APOBEC1 cDNA
Depositor|Developer Tsukahara, Toshifumi |
Other clones in our bank
External Database
Bacteriophage MS2 coat protein
    human APOBEC1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)。
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          RDB19360 pCS2+MT-MS2HB-APOBEC1 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Bhakta, S., RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code. Sci Rep. 2020 Oct 14;10(1):17304 (2020). PMID 33057101.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Bhakta, S., RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code. Sci Rep. 2020 Oct 14;10(1):17304 (2020). PMID 33057101.

          2022.05.16

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