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pCS2+MT-MS2HB-APOBEC1 (#RDB19360)

Expression vector of the catalytic domain of human APOBEC1 tagged with MS2HB at N-terminus for artificial enzymatic RNA editing system.

Clone info. Expression vector of the catalytic domain of human apolipoprotein B mRNA editing enzyme catalytic subunit 1 (APOBEC1) tagged with MS2HB at N-terminus for artificial enzymatic RNA editing system. Please refer to Bhakta, S. et al. Sci. Rep. 10 (1): 17304, 2020. MS2HB, bacteriophage MS2 coat protein dimer fused to a tag composed of two hexahistidine tags, a TEV cleavage site, and a signal sequence for in vivo biotinylation-tag (HTBH-tag). No myc-tag.
Comment Expression was confirmed by the depositor with microscopy.
Vector backbone pCS2 + MT
(plasmid)
Size of vector backbone 4.4 kb
Selectable markers Ampicillin
Gene/insert name Bacteriophage MS2 coat protein cDNA human APOBEC1 cDNA
Depositor|Developer Tsukahara, Toshifumi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19360 pCS2+MT-MS2HB-APOBEC1 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCS2+MT-MS2HB-APOBEC1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19360).

Reference section:

Bhakta, S., Sakari, M., Tsukahara, T., RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code. Sci. Rep. 2020 Oct 14;10(1):17304 (2020). PMID 33057101. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Bhakta, S., RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code. Sci. Rep. 2020 Oct 14;10(1):17304 (2020). PMID 33057101. [link to RRC of NBRP]