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pMRX-bsr-FLAG-OsTIR1 (#RDB19431)

Retroviral expression vector of oryza sativa TIR-L1 tagged with FLAG.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hatoyama, Y., J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Retroviral expression vector of oryza sativa F-box transport inhibitor response 1 (TIR-L1) tagged with FLAG at N-terminus.
Vector backbone pMRX-IRES-bsr-FLAG (plasmid)
Selectable markers Ampicillin (E. coli), Blasticidin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name Oryza sativa TIR-L1 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hatoyama, Y. et al., J. Cell Sci.(2022), 135, jcs.259184)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hatoyama, Y. et al., J. Cell Sci.(2022), 135, jcs.259184)。

Catalog # Resource name Shipping form Fee
RDB19431 pMRX-bsr-FLAG-OsTIR1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMRX-bsr-FLAG-OsTIR1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19431).

Reference section:

Hatoyama, Y., Homma, Y., Hiragi, S., Fukuda, M., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hatoyama, Y., Establishment and analysis of conditional Rab1- and Rab5-knockout cells using the auxin-inducible degron system. J. Cell Sci. 134 (24): jcs259184 (2021). PMID 34817057. [PubMed] [Article] [RRC of NBRP]