RDB19551 - pBS2SK+IA-ORF2

pBS2SK+IA-ORF2 (#RDB19551)

Plasmid vector of SARS-CoV2 cDNA encoding ORF2 (Spike protein).

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Clone info.	Plasmid vector of SARS-CoV2 cDNA encoding ORF2 (Spike protein). The cDNA was amplified from the genomic RNA of hCoV-19/Japan/NGS-IA-1/2020 (GISAID accession no. EPI-ISL-481251) by RT-PCR and cloned into the SalI site of pBluescript SK(+). Possible RNA synthesis using T7 and T3 RNA polymerase.
Comment	It can be liberated by SalI and SmaI or XbaI double digestion
Vector backbone	pBluescript II SK (+) (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Ampicillin
Growth conditions	LB+Amp, 37℃
Gene/insert name	SARS-CoV-2 ORF2 cDNA
Depositor\|Developer	DNA Bank, \| Nakade, Koji \|

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the NBRP Human Pathogenic Viruses as the source of the BIOLOGICAL RESOURCE in any publication. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to obtain approval from the Institute of Tropical Medicine, Nagasaki University.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The RECIPIENT agrees to expressly describe the acknowledgement of the NBRP Human Pathogenic Viruses as the source of the BIOLOGICAL RESOURCE in any publication. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to obtain approval from the Institute of Tropical Medicine, Nagasaki University.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースを使用して研究成果を発表する場合、NBRPヒト病原ウイルスから提供されたことを明記する。本件リソースを非営利機関が非営利目的の教育・研究用に用いる場合以外は、長崎大学熱帯医学研究所の許諾を必要とする。

Catalog #	Resource name	Shipping form	Fee
RDB19551	pBS2SK+IA-ORF2	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge