Please review the QC test results indicated by icon below as well as clone information before placing your order.

tdoxStayGold/pcDNA3

Fluorescent protein tdoxStayGold for fusion to the C-terminus of target molecule

Sequence (full)	RDB19611zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link]
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Hirano, M. et al., Nat. Biotechnol. doi: 10.1038/s41587-022-01278-2, 2022. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19611_B2Eap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'((GGGGS)x3,tdoxStayGold 5') Sequence file: RDB19611_B2Eaa.seq
>D08836A7_B2Ea_2_CMV_Forward_A06_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT GNNGNNAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT   121 ACGACTCACT ATAGGGAGAC CCAAGCTTGG TACCGGATGG TGGAGGCGGT TCAGGCGGAG   181 GTGGCTCTGG CGGTGGCGGA TCGATCAAGC TTATCGATAC CGTCGACCTC GAGATGGTGT   241 CTACAGGCGA GGAGCTGTTT ACCGGCGTGG TGCCCTTCAA GTTCCAGCTG AAGGGCACCA   301 TCAACGGCAA GAGCTTCACC GTGGAAGGCG AGGGCGAGGG CAATAGCCAC GAGGGCAGCC   361 ACAAAGGCAA GTACGTGTGC ACCAGCGGCA AACTGCCAAT GTCTTGGGCC GCCCTGGGAA   421 CTAGCTTCGG CTATGGCATG AAGTACTACA CCAAGTACCC CAGCGGCCTG AAGAACTGGT   481 TCCACGAGGT GATGCCCGAG GGCTTCACCT ACGACAGACA CATCCAGTAC AAGGGCGACG   541 GCAGCATCCA CGCCAAGCAC CAGCACTTCA TGAAGAACGG CACCTACCAC AACATCGTGG   601 AGTTCACCGG CCAGGACTTC AAGGAGAACA GCCCCGTGCT GACCGGCGAC ATGAACGTGA   661 GCCTGCCCAA CGAGGTGCAG CACATCCCCA GAGATGACGG CGTGGAGTGC CCAGTGACCC   721 TGCTGTACCC TCTGCTGANC GACAAGAGCA AGTGCGTGGA GGCCTACCAG AACACCATCA   781 TCAAGCCCCT GCACAATCAG CCAGCCCCCG ATGTGCCATA CCACTGGATC AGAAAGCAGT   841 ACACCCAGAN CAAGGANGAN ACCGANGNNA NAGANCACAT CATCCAGANC GAGANCCTGG   901 ANGNCCACCT GNNNTGGCNC GANCCTTCNG CTTCNGNCGN GGAATTCANN GCTGGTGGNT   961 CTGCTNGANN NANNGNNNGN NNTNNNNCNN GGGGNNNCGN NNNNGNNTCC NNCNNCNCNN 1021 GCNNNNNNNN NN //
Primer: BGH_rev2 (Pr0606) Region: insert 3'(tdoxStayGold 3') Sequence file: RDB19611_B2Eab.seq
>D08836A7_B2Ea_2_BGH_rev2_D06_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GNCANNNNNN NNANGGCTGG CAACTAGNNA    61 GGCACNNGTC GAGGCTNNNN NNNNGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC   121 CCTCTAGATG CATGCTCGAG CGGCCGCTTA TAAGTGTGCT TCGAGAGTCT CTGATTGAAT   181 AATATGATCA CGTTCCTCGG TATCATCTTT GCTTTGTGTG TATTGTTTAC GAATCCAGTG   241 ATATGGGACA TCAGGTGCTG GTTGATTATG AAGAGGCTTG ATGATTGTAT TTTGGTAAGC   301 CTCAACGCAT TTTGATTTAT CCGATAATAA AGGATAAAGC AAGGTCACTG GGCATTCTAC   361 TCCATCATCT CTGGGTATAT GTTGAACTTC ATTCGGTAAT GAGACATTCA TATCTCCAGT   421 TAAGACTGGA CTATTTTCTT TAAAATCCTG ACCAGTAAAT TCAACAATGT TGTGATAAGT   481 CCCATTTTTC ATAAAGTGTT GGTGTTTTGC ATGGATACTC CCATCGCCTT TATATTGAAT   541 ATGACGATCG TAGGTAAAGC CTTCAGGCAT TACTTCATGA AACCAGTTCT TCAGTCCACT   601 AGGATATTTG GTATAATATT TCATTCCATA ACCAAAGGAT GTTCCAAGTG CTGCCCATGA   661 CATCGGTAGT TTTCCACTTG TACAAACATA TTTTCCTTTA TGAGAACCTT CATGTGAATT   721 TCCTTCACCT TCGCCTTCAA CGGTAAACGA TTTGCCATTG ATGGTTCCTT TAAGTTGAAA   781 TTTAAATGGC ACCACCCCGG TGAACAGCTC CTCCCCTGTA CTCACCATAA GCTTCCCACC   841 GGCGCTACCG CCGGCACTCC CTCCGGNGGA NNCGCCAGCA NANCCACCTG CAGATGTGGA   901 NCCTCNNGCG CTNNCACCTG CNCTTNCAGA NCCCCCGGCA GANCCANCNG CGCTNCCANC   961 AGNNGNANNN TNCTGCNCTN CCCCCAGNNN ANGNNNTTNC NNNNGNNANA NNCNNCNGNN 1021 NGNNNCNGNN NNNNNNNN //
Primer: hStayGold_F1 (Pr1213) Region: insert mid Sequence file: RDB19611_B2Eac.seq
>D08836A7_B2Ea_2_hStayGold_F1_B06_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNCCTGCC NNNCNAGGTG CAGCACATCC CCAGAGATGA    61 CGGCGTGGAG TGCCCAGTGN CCCTGCTGTA CCCTCTGCTG AGCGACAAGA GCAAGTGCGT   121 GGAGGCCTAC CAGAACACCA TCATCAAGCC CCTGCACAAT CAGCCAGCCC CCGATGTGCC   181 ATACCACTGG ATCAGAAAGC AGTACACCCA GAGCAAGGAC GACACCGAGG AGAGAGACCA   241 CATCATCCAG AGCGAGACCC TGGAGGCCCA CCTGCCATGG CACGAGCCTT CTGCTTCTGC   301 CGTGGAATTC AGTGCTGGTG GTTCTGCTGG AGGAAGCGCC GGAGGTTCCG CTGGGGGAAG   361 CGCAGGCGGC TCCGGCAGCG CTGGCGGGAG TGCCGGGGGC AGTACCTCCG CAGGAGGAAG   421 TGCAGGGGGA AGCGCTGGTG GCAGTGCGGG GGGTTCCGCA GGCGGTAGCG GTTCCGCCGG   481 TGGATCTGCC GGTGGAAGCA CATCTGCTGG GGGTAGTGCA GGAGGGTCCG CTGGTGGGAG   541 CGCAGGTGGA TCTGCCGGGG GGTCTGGAAG TGCAGGTGGT AGCGCTGGAG GGTCCACATC   601 TGCAGGTGGG TCTGCTGGCG GTTCCGCCGG AGGGAGTGCC GGCGGTAGCG CCGGTGGGAA   661 GCTTATGGTG AGTACAGGGG AGGAGCTGTT CACCGGGGTG GTGCCATTTA AATTTCAACT   721 TAAAGGAACC ATCAATGGCA AATCGTTTAC CGTTGAAGGC GAANGTGAAG GAAATTCACA   781 TGAAGGTTCT CATAAAGGAA AATATGTTTG TACAAGTGGA AAACTACCGA TGTCATNNNN   841 AGCACTTGNA ACATCCTTTG GTTATGGAAT GAAATATTAT ACCAAATATC CTAGTGNANT   901 GAAGAACTGG TTTCATGAAG TAATGCCTGN ANNNNTTTAC CTACNATCGN CATATTCNNT   961 ATAAAGGNNA TGGGANTATC CNTGCNAAAC NCCNNCNCTT TATGAAAAAT GGGNNNTATC 1021 NNNNNNNNNN //
Primer: EV_linker_F1 (Pr1214) Region: insert mid Sequence file: RDB19611_B2Ead.seq
>D08836A7_B2Ea_2_EV_linker_F1_D10_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNNNNNCGC ANGTGGNNCT GCCGGGGGGT CTGGAAGTGC    61 AGGTGGTAGC GCTGGAGGGT CCACATCTGC AGGTGGGTCT GCTGGCGGTT CCGCCGGAGG   121 GAGTGCCGGC GGTAGCGCCG GTGGGAAGCT TATGGTGAGT ACAGGGGAGG AGCTGTTCAC   181 CGGGGTGGTG CCATTTAAAT TTCAACTTAA AGGAACCATC AATGGCAAAT CGTTTACCGT   241 TGAAGGCGAA GGTGAAGGAA ATTCACATGA AGGTTCTCAT AAAGGAAAAT ATGTTTGTAC   301 AAGTGGAAAA CTACCGATGT CATGGGCAGC ACTTGGAACA TCCTTTGGTT ATGGAATGAA   361 ATATTATACC AAATATCCTA GTGGACTGAA GAACTGGTTT CATGAAGTAA TGCCTGAAGG   421 CTTTACCTAC GATCGTCATA TTCAATATAA AGGCGATGGG AGTATCCATG CAAAACACCA   481 ACACTTTATG AAAAATGGGA CTTATCACAA CATTGTTGAA TTTACTGGTC AGGATTTTAA   541 AGAAAATAGT CCAGTCTTAA CTGGAGATAT GAATGTCTCA TTACCGAATG AAGTTCAACA   601 TATACCCAGA GATGATGGAG TAGAATGCCC AGTGACCTTG CTTTATCCTT TATTATCGGA   661 TAAATCAAAA TGCGTTGAGG CTTACCAAAA TACAATCATC AAGCCTCTTC ATAATCAACC   721 AGCACCTGAT GTCCCATATC ACTGGATTCG TAAACAATAC ACACAAAGCA AAGATGANAC   781 CGAGGAACGT GATCATATTA TTCAATCNGA GNCTCTCGAA GCACACTTAT AAGCGGCCGC   841 TCGANCATGC NTCTAGAGGG NCCTATTCTA TAGTGTCACC TAAATGCTAN AGCTCGCTGA   901 NCAGCCTCNA CTGTGCCTTC TANTTGNCNG CCNTCTGNTG TTTGCCCNNC CCCCNNNCCT   961 TNNNTGNNCC NNNNANNGNG CCNCNNCCNC TGNCCTTNNC NANNAAAATG NNNAANNNGC  1021 NTCGNNNTNN NTGANTNNNN GNCATTCNNN NTNNNNGGNG NNNNNNNNNG GGNNNNNNNN 1081 NN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB19611_B2Eae.seq
>D08836A7_B2Ea_2_SV40pro_ori_F_E06_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNG CCTCTGCCTC TGANCTATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG   181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG   241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA   301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT   361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA   421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC   481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC   541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC   601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT   661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA   721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGNNCGCTTT TCTGGATTCA TCGACTGTGG   781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACNCGTG ATATTGCTGA   841 AGANCTTGGC GGCGAATGGG CTGACCGCTT CCTCGNGCTT TACGGTATCG CCGCTCCCGA   901 TTCNCAGCGC ATCGCCTTCN ATCGCCTTCT TGANNANTTC TTCTGANCGG GNNNCTGGGG   961 TTCGAAATGA NCNANCNANC GANGCCNNAN CNNGNNNTCN CGANATTTCN NNTNCNNCNN 1021 CNNNCNNNCN ANNAANNNNN GGGNTTNNGN NNNNNNTTTN NNGNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.01.29

HTML_filter_RDB_240127.pl