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tdoxStayGold/pcDNA3 (#RDB19611)

Fluorescent protein tdoxStayGold for fusion to the C-terminus of target molecule


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hirano, M., Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. For fusion of tdoxStayGold to the C-terminus of a target molecule (X). tdoxStayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold, c4, EV linker, n1, and oxStayGold. The gene for the former oxStayGold has been resynthesized with mammalian-preferred codons, and the gene for the latter oxStayGold has the jellyfish original codons. n1 and c4 are the appendages that facilitate fusions to the N- and C-termini of oxStayGold, respectively. X-tdoxStayGold is required to terminate with the original C-terminus of oxStayGold. oxStayGold is a variant of StayGold with two mutations: C174I and C208I; oxStayGold is well expressed also in the cytosolic fraction as StayGold. See Hirano, M. et al., Nat. Biotechnol. 2022 doi: 10.1038/s41587-022-01278-2 for more information.
Comment The accession numbers in the DDBJ/EMBL/GenBank databases are LC601652 for StayGold, LC593679 for h-StayGold and LC601653 for oxStayGold.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell).
Growth conditions LB+Amp, 37oC
Gene/insert name Cytaeis uchidae GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |

Distribution information

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Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Hirano, M. et al., Nat. Biotechnol. 40 (7): 1132-1142 (2022).
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MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Hirano, M. et al., Nat. Biotechnol. 40 (7): 1132-1142 (2022).
備考 提供条件は英文のまま記載してください。

Catalog # Resource name Shipping form Fee
RDB19611 tdoxStayGold/pcDNA3 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The tdoxStayGold/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19611).

Reference section:

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., Inaura, S., Matsumura, Y., Hama, H., Okada, Y., Fujiwara, T., Morimoto, T., Katayama, K., Miyawaki, A., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirano, M., A highly photostable and bright green fluorescent protein. Nat. Biotechnol. 40 (7): 1132-1142 (2022). PMID 35468954. [PubMed] [Article] [RRC of NBRP]