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pMXs-IP-EGFP-hATG13 (#RDB19625)

Retroviral vector for stable expression of human ATG13 with N-terminal EGFP.

Clone info. Retroviral vector for stable expression of human ATG13 with N-terminal EGFP.
Vector backbone pMXs-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human ATG13; Aequorea victoria GFP.
Depositor|Developer Mizushima, Noboru |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Hosokawa, N. et al. Mol. Biol. Cell, 20(7), 1981-1991, 2009)
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hosokawa, N. et al. Mol. Biol. Cell, 20(7), 1981-1991, 2009)。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19625 pMXs-IP-EGFP-hATG13 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IP-EGFP-hATG13 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19625).

Reference section:

Hosokawa, N., Hara, T., Kaizuka, T., Kishi, C., Takamura, A., Miura, Y., Iemura, S., Natsume, T., Takehana, K., Yamada, N., Guan, J.L., Oshiro, N., Mizushima, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19829_B2Lgp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(EGFP 5')
Sequence file: RDB19829_B2Lga.seq check
>D08693C3_B2Lg_3_MMLV-pol-F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGNNTNNNNN CTTGGNNACN CGCCGCCCNC GTGAAGGCTG
   61 CCGACCCCGG GGGTGGACCA TCCTCTAGAC TGCCGGATCT AGCTAGTTAA TTAAGGATCC
  121 CAGTGTGGTG GTACGGGAAT TCCTGCAGGC CTCGAGGGCC GGCGCGCCGC GGCCGCCGCC
  181 ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
  241 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
  301 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
  361 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG
  421 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC
  481 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
  541 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG
  601 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
  661 AACGGNNTCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC
  721 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACNAC
  781 CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCN NCGAGAAGCG CGATCACATG
  841 GTCCTGCTGG ANNNCNNNAC CGCCGCCGGG ATCANTCTCN GNCATGGACN ANCTGTACAA
  901 GTCCNGGANT CNGATCTCNA GCTCAAGCTT CNNATTCTNN NNNNCNACGG TNNCGCGGGC
  961 CNNGNNNNCN NGGNANNTGA TNNNNATTNC NANGNNANAA NNNANCTNNN NNNNNTNATN
 1021 NNNTTTTTGC CNNNANANTG NCCNNNNGAN NGTCNNNGNN NNGCTNNNNA AANNNTTNNN
1081 NTCNNNCATN ANNNNNNNNN N
//
Primer: hATG13-C_F (Pr1278)
Region: insert 3'(hATG13 3'),IRES
Sequence file: RDB19829_B2Lgb.seq check
>D08692C3_B2Lg_3_hATG13-C_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGTTTGC TCCNNNGAAT TTGGAGCTGG AGGANACCGA
   61 TCCAATGGTG AATCCTCCNN ANTCCCCAGA GACTGAATCT CCTCTCCAGG GCAGCCTGCA
  121 CTCAGATGGC TCCAGCGGGG GCAGCAGTGG CAATACCCAT GATGACTTTG TTATGATAGA
  181 CTTTAAACCA GCTTTTTCTA AAGATGACAT TCTTCCGATG GACCTGGGGA CCTTCTATCG
  241 GGAGTTTCAG AACCCACCTC AGCTGAGCAG CCTCTCCATA GATATTGGAG CACAGTCCAT
  301 GGCTGAAGAC TTGGACTCAT TACCAGAGAA GCTGGCTGTG CATGAGAAGA ATGTCCGCGA
  361 GTTTGATGCC TTTGTGGAAA CCCTGCAGGG ATCCACCGGA TCTAGATAAC TGATCATAAT
  421 CAGCCATACC ACATTTGTAG GCGGCCGCTA CGTAAATTCC GCCCCCCCCC CCCCTCTCCN
//
Primer: FP-N_F1 (Pr0920)
Region: insert mid(EGFP 3',hATG13 5')
Sequence file: RDB19829_B2Lgc.seq check
>D08693C3_B2Lg_3_FP-N_F1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNNNGAAG CAGCACGACT TCTTCNAGTC
   61 CGCCNTGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA
  121 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA
  181 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA
  241 CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
  301 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC
  361 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC
  421 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC
  481 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTCC GGACTCAGAT CTCGAGCTCA
  541 AGCTTCGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA TCCATGGAAA CTGATCTCAA
  601 TTCCCAGGAC AGAAAGGACC TGGACAAGTT TATTAAATTT TTTGCCCTCA AGACTGTCCA
  661 AGTGATTGTC CAGGCTCGGC TTGGTGAAAA GATTTGCACT CGTTCATCAT CTTCTCCAAC
  721 GGGTTCAGAT TGGTTCAACT TAGCAATCAA AGACATCCCA GAGGTTACAC ATGAAGCAAA
  781 GAAGGCNCTG GCAGGACAGC TGCCTGCAGT CGGGAGGNCC NNTGTGTGTG GANNTTTCAC
  841 TTANGACTTC TGANGGAGNN NNCNNTGGAN NTGGAAATAT GGTGTCTTGA AATGAATGAA
  901 AANTGTGATN AAGAAATCAA AGTTTCCNAN NCNGTGTACA ACANANNGTC NNTGCTGCTG
  961 AANNNCNNNC TTGCTATANC TAGGGNGACA CCNNNCNNNN NNNNTCTCCA NNAAACNNGG
 1021 NCNTGNATAT GTNNTATTNN ANNNGNNNNN NNNTTNGGNN NNANTTCNNC TGANNGNCNT
1081 NNNNANNNNN NTNCNNNNNN TNNNNNNNNG GGNNNNNNNN NN
//
Primer: EGFP-C (Pr0025)
Region: insert mid(hATG13 5')
Sequence file: RDB19829_B2Lgd.seq check
>D08693C3_B2Lg_3_EGFP-C_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNAN NNTNNNNNNN NNCCGGANTC NGATCTCGAG
   61 CTCAAGCTTC GAATTCTGCA GTCGACGGTA CCGCGGGCCC GGGATCCATG GAAACTGATC
  121 TCAATTCCCA GGACAGAAAG GACCTGGACA AGTTTATTAA ATTTTTTGCC CTCAAGACTG
  181 TCCAAGTGAT TGTCCAGGCT CGGCTTGGTG AAAAGATTTG CACTCGTTCA TCATCTTCTC
  241 CAACGGGTTC AGATTGGTTC AACTTAGCAA TCAAAGACAT CCCAGAGGTT ACACATGAAG
  301 CAAAGAAGGC ACTGGCAGGA CAGCTGCCTG CAGTCGGGAG GTCCATGTGT GTGGAGATTT
  361 CACTTAAGAC TTCTGAGGGA GATTCCATGG AGCTGGAAAT ATGGTGTCTT GAAATGAATG
  421 AAAAGTGTGA TAAAGAAATC AAAGTTTCCT ACACGGTGTA CAACAGACTG TCATTGCTGC
  481 TGAAGTCCCT TCTTGCTATA ACTAGGGTGA CACCAGCCTA TAGGCTCTCC AGGAAACAAG
  541 GGCATGAATA TGTCATATTA TACAGGATAT ATTTTGGAGA AGTTCAGCTG AGTGGCTTAG
  601 GAGAAGGCTT CCAGACAGTT CGTGTTGGGA CAGTGGGCAC CCCTGTGGGC ACCATCACTC
  661 TTTCTTGTGC TTANNNAATT AACTTGGCAT TCATGTCTAC CAGGCAATTT GAGAGGACCC
  721 CACCTATCAT GGGGATTATT ATTGATCACT TTGTGGACCG TCCCTATCCC AGCTCCTCTC
  781 CCATGCACCC CTGCAATTAC NGAACTGCTG GNGAGGACAC TGGANTAATA TACCCGTCTG
  841 TAGAAGANTC TCAAGAAGTG TGNNNCNNCT CTTTTNNCAC CTCCCCACNN NCNCAGCNCT
  901 CAAGCTCTCN CNTTTNCCNA TNNNCNNGCT NCCCTGGGCG NNGNNNNCAN CNGNNCNNGN
  961 NTTANCCNGN ANNGNTNNCN NNNNGNCTNN AATGNNNNNN ACCCTCNCCA NCTGNNGGNT
 1021 TNCNNNNNAN NNANNNNGGG GNNNNNNCTN GNNCNNACNA NNCNNGNNCA NNGNACCNNN
1081 NNNNNNN
//
Primer: MMLV psi_R1 (Pr1054)
Region: 5' LTR
Sequence file: RDB19829_B2Lge.seq check
>D08693C3_B2Lg_3_MMLV psi_R1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGTTGCT GGCCNGCNNN
   61 NNNCCCGNNT GGTGGGTCGG TGGNCCNNGN NNNNGGGTCT CCCGATCCCG GACGAGCCCC
  121 CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAN AGGAGACCCT CCCAAGGAAC
  181 AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG
  241 CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG
  301 CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA
  361 AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGAA
  421 ACTGCTGAGG GCTGGACCGC ATCTGGGGAC CATCTGTTCT TGGCCCTGAG CCGGGGCAGG
  481 AACTGCTTAC CACAGATATC CTGTTTGGCC CATATTCNNC TGTNNNNNNT GTTCTTGGCC
  541 CTGAGCCGGG GCAGGAACTG CTTACCACAG ANNNNNTGTT TGGCCCATAT TNNNNNNTTC
  601 CATCTGTTCC TGACCTTGAT CTGAACTTTT CTATTCTCAG TTNTGTATTT TTCCATGCNN
  661 NNNNNNATGG CGTTACTTAN NCTAGCTTGC CNAACCTANN NGTGGGGTCT TTCATTATNC
721 NNGTGGCACT TTTCNNNNAA ATGTGCGCGG AACCCCTATT TNNNNNNNNN N
//
Primer: MMLV psi_F (Pr0863)
Region: MMLV psi,gag,pol region
Sequence file: RDB19829_B2Lgf.seq check
>D08693C3_B2Lg_3_MMLV psi_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNN NNNNNNNNTT TCGGTTTGGG ANCGAAGCCG
   61 CGCCGCGCGT CTTGTCNNCT GNNNCATCGT TCTGTGTTGT CTCTGTCTGA CTGTGTTTCT
  121 GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC TTAAGTTTGA CCTTAGGTCA
  181 CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA GATGTCAAGA AGAGACGTTG
  241 GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC GGATGGCCGC GAGACGGCAC
  301 CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG
  361 ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC
  421 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT CCGCCCCGTC
  481 TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC TCCCTTTATC CAGCCCTCAC
  541 TCCTTCTCTA GGCGCCCCCA TATGGCCATA TGAGATCTTA TATGGGGCAC CCCCGCCCCT
  601 TGTAAACTTC CCTGACCCTG ACATGACNNN ANTTACTAAC AGCCCCTCTC TCCAAGCTCA
  661 CTTNNNNGCT CTCTACTTAG TCCANCNCGA AGTCTGGAGA NNNNNGGCGG CAGCCTACCA
  721 AGAACANNNN NNCCGACCGG NGGNNNCTCA CCCTTANCGA GTCGGCNANN NNNNGTGGGT
  781 CCGCCGANAC CANANNNNNA ANCNNNAACC TCGCTGGAAA GGANCTTACN NNNTCCTGCT
  841 GANCACCCNN NNNNNCCCTC NAANNANANN GNNTCNCANC TNGNNNNCNN NNCNNCCCAC
901 NTNAANGNTG NCNNNCNNNG NGGNNNNNNN NNN
//
Primer: IRES-F (Pr0036)
Region: IRES,puroR
Sequence file: RDB19829_B2Lgg.seq check
>D08693C3_B2Lg_3_IRES-F_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNGTNNNNNT NNNNNNNNNN TCTAGGCCCC CCGAACCACG
   61 GGGANGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTTACCGAG
  121 TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC
  181 GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC
  241 GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG
  301 GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA
  361 GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG
  421 GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG
  481 TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC
  541 GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC
  601 GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG
  661 GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGTCGACGAT
  721 AAAATAAAAG ANTTTATTTA GTCTCCNGAA AAAGGGGGGA ATGNAAAGAC CCCACCTGTA
  781 GGTTTGGCAA GCTAGCTNAA NTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATNACTGA
  841 NAATANAGAA NTTCANATCN NGGTCAGGAA NNNATNGNAC AGCTGAATNT GGGCCNNACA
  901 GGATATCTGT GGTAAGCAGT TNCTGNCCCG GCNNNNGNCC AANAACNGAT GGAACAGCTG
  961 AATATGGNNN NNANNNGANN TCNGTGGTAN NCAGTTNCTN NCCCNGNTNN NGNCCNANNN
 1021 NNNATNGNNN NNNNNANGNN GNCNGNNNNN NNNNANTTTN NNNNNNNAAN NANNNNANNG
1081 TTNNNNNNGG GNNNNNCCCN NNGNNNNNNN ANNNNNCCNN NNNNNNNN
//
Primer: PuroR_F (Pr0554)
Region: 3' LTR
Sequence file: RDB19829_B2Lgh.seq check
>D08693C3_B2Lg_3_PuroR_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNC GCGCACCTGG TGCNTGACCC
   61 GCAAGCCCGG TGCCTGNNTC NANGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG
  121 GGGGGAATGA AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC
  181 AAGGCATGGA AAAATACATA ACTGAGAATA GAGAAGTTCA GATCAAGGTC AGGAACAGAT
  241 GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
  301 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
  361 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAN CCCTCAGCAG
  421 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
  481 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
  541 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC
  601 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC
  661 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCNNNGGGGG TCTTTCACAT
  721 GCANNNNGNA TCNAAATTAA TTTGGTTTTT TTTCTTAAGT ATTTACNTTA AATGGCCATA
  781 GTTGCNTTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGGCGCTCTT
  841 CCGCTTCCTC GCTCNCTGAC TCGCTGCNCT CGGTCGTTCN GGCTGCNGNN ANNNNNNNNC
  901 NGCTNNCTCN AANGCGGGTA ATNNNGGTNA TCNNCANNAT CNGGGGATAN NNNANGAAAA
  961 GAACATGTGA NCNAANNGNC NNNNNAANGN NNNNNANCNN TAAAANNGNC NNNNNTNNCT
1021 NGGCNNTTTT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hosokawa, N., Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy. Mol. Biol. Cell 20 (7): 1981-1991 (2009). PMID 19211835. [link to RRC of NBRP]