RDB19682 - pcmvT7-KpnI-polyA

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pcmvT7-KpnI-polyA (#RDB19682)

Infusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial high copy number plasmid (pCDNA3 backbone).

Alternative name	pT7-KpnI-pA
Clone info.	Infusion/Gibson assembly platform vector for dual ussage mammalian expression and T7 In Vitro Transcription. Bacterial high copy number plasmid (pCDNA3 backbone).
Comment	delivative of T7-NLS hCas9-pA (RDB13130), (Yoshimi, K. et al. Nat. Commun. 7: 10431, 2016).
Vector backbone	pCDNA3 (plasmid)
Growth conditions	LB+amp, 37dC
Growth remarks	Recommended host, DH5alpha
Depositor\|Developer	DNA Bank, \| Nakashima, Kenichi \|

Sequence (full)	RDB19682hts01.seq Assembled from experimentally sequenced data.
Publication	Unpublished bioresource
Test sheet	RDB19682C0p1-1.pdf

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19682	pcmvT7-KpnI-polyA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge