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pMRXIP-GFP-hATG2A

Retroviral vector for stable expression of human ATG2A with N-terminal GFP.

Catalog number RDB19782
Resource name pMRXIP-GFP-hATG2A
Clone info. Retroviral vector for stable expression of human ATG2A with N-terminal GFP.
Vector backbone pMRX-IP (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell)
Gene/insert name human ATG2A cDNA; Aequorea victoria GFP (EGFP).
Depositor|Developer Mizushima, Noboru |
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External Database
human ATG2A

          Reference sequence
            
           
          Sequence (full) RDB19782hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19782_B2Jlp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tamura, N. et al. FEBS Lett. 591(23): 3819-3830, 2017)
          Additional terms and conditions:
          Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tamura, N. et al. FEBS Lett. 591(23): 3819-3830, 2017)。
          付加的提供条件:
          pMRXベクターについて:利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19782 pMRXIP-GFP-hATG2A DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pMRXIP-GFP-hATG2A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19782).

          Reference section:

          Tamura, N., Nishimura, T., Sakamaki, Y., Koyama-Honda, I., Yamamoto, H., Mizushima, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          References

          Original, user report and related articles

          original Tamura, N., Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets. FEBS Lett. 591 (23): 3819-3830 (2017). PMID 29113029. [link to RRC of NBRP]

          2023.09.04

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