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pcDNA3-GFP-LC3-RFP-LC3 deltaG (#RDB19804)

A autophagic flux probe, expression vector of GFP-rat LC3-RFP-rat LC3 deletion G.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kaizuka, T., Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. A autophagic flux probe, expression vector of GFP-rat LC3-RFP-rat LC3 deletion G. See graphical abstract of Kaizuka, T. et al., Mol. Cell, 64 (4): 835-849, 2016.
Vector backbone pcDNA3-GFP (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name rat Map1lc3b cDNA; Aequorea victoria GFP (EGFP); Discosoma sp. RFP (mRFP).
Depositor|Developer Mizushima, Noboru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kaizuka, T. et al. Mol. Cell. 64(4): 835-849, 2016)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kaizuka, T. et al. Mol. Cell. 64(4): 835-849, 2016)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee
RDB19804 pcDNA3-GFP-LC3-RFP-LC3 deltaG DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3-GFP-LC3-RFP-LC3 deltaG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19804).

Reference section:

Kaizuka, T., Morishita, H., Hama, Y., Tsukamoto, S., Matsui, T., Toyota, Y., Kodama, A., Ishihara, T., Mizushima, T., Mizushima, N., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [PubMed] [Article] [RRC of NBRP]