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pcDNA3-GFP-LC3-RFP-LC3 deltaG

A autophagic flux probe, expression vector of GFP-rat LC3-RFP-rat LC3 deletion G.

Catalog number RDB19804
Resource name pcDNA3-GFP-LC3-RFP-LC3 deltaG
Clone info. A autophagic flux probe, expression vector of GFP-rat LC3-RFP-rat LC3 deletion G. See graphical abstract of Kaizuka, T. et al., Mol. Cell, 64 (4): 835-849, 2016.
Vector backbone pcDNA3-GFP (plasmid)
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name rat Map1lc3b cDNA; Aequorea victoria GFP (EGFP); Discosoma sp. RFP (mRFP).
Depositor|Developer Mizushima, Noboru |
Other clones in our bank
External Database
rat Map1lc3b
          Reference sequence
            
           
          Sequence (full) RDB19804hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB19804_B2Jlp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kaizuka, T. et al. Mol. Cell. 64(4): 835-849, 2016)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kaizuka, T. et al. Mol. Cell. 64(4): 835-849, 2016)。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19804 pcDNA3-GFP-LC3-RFP-LC3 deltaG DNA solution

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3-GFP-LC3-RFP-LC3 deltaG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19804).

          Reference section:

          Kaizuka, T., Morishita, H., Hama, Y., Tsukamoto, S., Matsui, T., Toyota, Y., Kodama, A., Ishihara, T., Mizushima, T., Mizushima, N., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          References

          Original, user report and related articles

          original Kaizuka, T., An Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849 (2016). PMID 27818143. [link to RRC of NBRP]

          2023.07.25

          GNP_filter3_RDBDEP_html_230702.pl