Resource data sheet
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SPOON/pcDNA3

Vector of SPOON, a spontaneously blinking fluorescent protein, for mammalian expression.

Catalog number RDB19863
Resource name SPOON/pcDNA3
Clone info. Vector of SPOON, a spontaneously blinking fluorescent protein, for mammalian expression.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP (SPOON).
Reference of insert sequence LC320161 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |
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External Database
Aequorea victoria GFP (SPOON)

            Reference sequence
              
             
            Sequence (full) RDB19863hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB19863_B3Acp1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Arai, Y. et al., ACS Chem. Biol. 13 (8): 1938-1943, 2018) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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            MTA, for use for not-for-profit academic purpose [Word]
            MTA, for use for for-profit purpose [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Arai, Y. et al., ACS Chem. Biol. 13 (8): 1938-1943, 2018)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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            提供同意書 (MTA, 非営利学術目的用)[Word]
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            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19863 SPOON/pcDNA3 DNA solution


            How to cite this biological resource

            Materials & Methods section:

            The SPOON/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19863).

            Reference section:

            Arai, Y., Takauchi, H., Ogami, Y., Fujiwara, S., Nakano, M., Matsuda, T., Nagai, T., Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. ACS Chem. Biol. 13 (8): 1938-1943 (2018). PMID 29963852. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            References

            Original, user report and related articles

            original Arai, Y., Spontaneously Blinking Fluorescent Protein for Simple Single Laser Super-Resolution Live Cell Imaging. ACS Chem. Biol. 13 (8): 1938-1943 (2018). PMID 29963852. [link to RRC of NBRP]

            2024.04.11

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