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gTEMP/pcDNA3

Vector of gTEMP, a genetically-encoded temperature indicator, for mammalian expression.

Catalog number RDB19869
Resource name gTEMP/pcDNA3
Clone info. Vector of gTEMP, a genetically-encoded temperature indicator, for mammalian expression.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Aequorea victoria GFP (Sirius), GFP (mT-Sapphire).
Reference of insert sequence LC132716 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |
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External Database
Aequorea victoria GFP (Sirius)
    Aequorea victoria GFP (mT-Sapphire)
            Reference sequence
              
             
            Sequence (full) RDB19869hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB19869_B3Acp1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nakano, M. et al., PLoS One 12 (2): e0172344, 2017) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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            MTA, for use for not-for-profit academic purpose [Word]
            MTA, for use for for-profit purpose [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nakano, M. et al., PLoS One 12 (2): e0172344, 2017)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19869 gTEMP/pcDNA3 DNA solution

            How to cite this biological resource

            Materials & Methods section:

            The gTEMP/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19869).

            Reference section:

            Nakano, M., Arai, Y., Kotera, I., Okabe, K., Kamei, Y., Nagai, T., Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLoS One 12 (2): e0172344 (2017). PMID 28212432. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Nakano, M., Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response. PLoS One 12 (2): e0172344 (2017). PMID 28212432. [link to RRC of NBRP]

            2024.04.11

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