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OeNL/pcDNA3

Vector of orange enhanced-nanolantern, a chemiluminescent protein, for mammalian expression.

Catalog number RDB19871
Resource name OeNL/pcDNA3
Clone info. Vector of orange enhanced-nanolantern, a chemiluminescent protein, for mammalian expression. For more information, see Fig.1 of Suzuki, K. et al., Nat. Commun., 7: 13718, 2016.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Oplophorus gracilirostris Luciferase (NanoLuc); Fungia concinna RFP (mKusabira-Orange).
Reference of insert sequence LC128717 (DDBJ accession number)
Depositor|Developer Nagai, Takeharu |
Other clones in our bank
External Database
Oplophorus gracilirostris Luciferase (NanoLuc)
    Fungia concinna RFP (mKusabira-Orange)
            Reference sequence
              
             
            Sequence (full) RDB19871hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB19871_B3Acp1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Suzuki, K. et al., Nat. Commun. 7: 13718, 2016) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Suzuki, K. et al., Nat. Commun. 7: 13718, 2016)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

            付加的提供条件：
            本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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            提供同意書 (MTA, 非営利学術目的用)[Word]
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            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19871 OeNL/pcDNA3 DNA solution

            How to cite this biological resource

            Materials & Methods section:

            The OeNL/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19871).

            Reference section:

            Suzuki, K., Kimura, T., Shinoda, H., Bai, G., Daniels, M.J., Arai, Y., Nakano, M., Nagai, T., Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7: 13718 (2016). PMID 27966527. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            References

            Original, user report and related articles

            original Suzuki, K., Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7: 13718 (2016). PMID 27966527. [link to RRC of NBRP]

            2023.10.05

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