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pCAGGS-myc-NES-shootin1a (261-377)

Expression vector to overexpress dominant negative human shootin1a (amino acids 261-377) tagged with myc and nuclear exclusion signal (NES) at N-terminus.

Catalog number RDB19943
Resource name pCAGGS-myc-NES-shootin1a (261-377)
Clone info. Expression vector to overexpress dominant negative human shootin1a (amino acids 261-377) tagged with myc and nuclear exclusion signal (NES) at N-terminus, which disrupts the interaction between shootin1a and cortactin. See Fig. 7A-C of Kubo, Y. et al., J. Cell Biol., 210(4): 663-676, 2015.
Vector backbone pCAGGS (plasmid)
Selectable markers Ampicillin (E. coli)
Gene/insert name human SHTN1 cDNA; synthetic Myc-tag, NES sequence.
Depositor|Developer Inagaki, Naoyuki |
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human SHTN1
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          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Kubo Y., et al. J Cell Biol. 210(4):663-76, 2015) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization　and by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献(Kubo Y., et al. J Cell Biol. 210(4):663-76, 2015)を引用し、謝辞の表明を必要とする。営利機関並びに学術機関の営利目的利用の希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB19943 pCAGGS-myc-NES-shootin1a (261-377) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCAGGS-myc-NES-shootin1a (261-377) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19943).

          Reference section:

          Kubo, Y., Baba, K., Toriyama, M., Minegishi, T., Sugiura, T., Kozawa, S., Ikeda, K., Inagaki, N., Shootin1-cortactin interaction mediates signal-force transduction for axon outgrowth. J. Cell Biol. 210 (4): 663-676 (2015). PMID 26261183. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

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          References

          Original, user report and related articles

          original Kubo, Y., Shootin1-cortactin interaction mediates signal-force transduction for axon outgrowth. J. Cell Biol. 210 (4): 663-676 (2015). PMID 26261183. [link to RRC of NBRP]

          2023.12.11

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