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pCAGGS-myc-shootin1a (30-146) (#RDB19945)

Expression vector to overexpress dominant negative rat shootin1a (amino acids 30-146) tagged with myc at N-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kastian, R.F., Cell Rep. 35 (7): 109130 (2021). PMID 34010643. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector to overexpress dominant negative rat shootin1a (amino acids 30-146) tagged with myc at N-terminus, which disrupts the interaction between shootin1a and N-cadherin. See Fig. 6C of Kastian, RF. et al., Cell Rep., 35(7): 109130, 2021.
Vector backbone pCAGGS (plasmid)
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Amp, 37oC
Gene/insert name rat Shtn1 cDNA; synthetic Myc-tag.
Depositor|Developer Naoyuki, Inagaki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ria Fajarwati Kastian et al. Cell Rep. 35(7):109130, 2021) and an acknowledgment to the DEPOSITOR are requested. For use of the BIOLOGICAL RESOURCE by a for-profit organization　and by a not-for profit organization for a for-profit research, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献(Ria Fajarwati Kastian et al. Cell Rep. 35(7):109130, 2021)を引用し、謝辞の表明を必要とする。営利機関並びに学術機関の営利目的利用の希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。

Catalog # Resource name Shipping form Fee
RDB19945 pCAGGS-myc-shootin1a (30-146) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAGGS-myc-shootin1a (30-146) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19945).

Reference section:

Kastian, R.F., Minegishi, T., Baba, K., Saneyoshi, T., Katsuno-Kambe, H., Saranpal, S., Hayashi, Y., Inagaki, N., Shootin1a-mediated actin-adhesion coupling generates force to trigger structural plasticity of dendritic spines. Cell Rep. 35 (7): 109130 (2021). PMID 34010643. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kastian, R.F., Shootin1a-mediated actin-adhesion coupling generates force to trigger structural plasticity of dendritic spines. Cell Rep. 35 (7): 109130 (2021). PMID 34010643. [PubMed] [Article] [RRC of NBRP]