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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCriMGET_9-12a

Plasmid vector to construct a donor cassette for knock-in of long external DNA cleaved by both CRISPR-Cas9 and CRISPR-Cas12a.

Catalog number RDB20013
Resource name pCriMGET_9-12a
Clone info. Plasmid vector to clone a targeting cassette. By using Syn-crRNA-TS-sgRNA with Cas9 nuclease, or Syn-crRNA-TS-crRNA with Cas12a, the targeting cassette can be excised in vivo. See Fig.1 of Ishibashi, R. et al., Sci. Rep., 12(1): 17775, 2022.
Vector backbone pBluescript II SK(+) (plasmid)
Selectable markers Ampicillin
Growth remarks NEB Stable or an equivalent host is recommended for amplifying this clone.
Gene/insert name synthetic sequence (Syn-crRNA-TS_9-12a)
Depositor|Developer Ishibashi, Riki |
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            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 12(1): 17775, 2022)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること (Ishibashi, R. et al., Sci. Rep. 12(1): 17775, 2022)。
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            RDB20013 pCriMGET_9-12a DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pCriMGET_9-12a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20013).

            Reference section:

            Ishibashi, R., Maki, R., Kitano, S., Miyachi, H., Toyoshima, F., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB20013_B3Gkp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: f1ori_R4 (Pr1179)
            Region: T7 pro,syn-crRNA-TS_9-12a.1,MCS
            Sequence file: RDB20013_B3Gka.seq check
            >D09326A1_B3Gk_1_f1ori_R4_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCGNCNC NNCNGGNNGC GTCCCATTCN
               61 CCATTCAGGC TGCGCAACTG TTGGGAANNN NNNNNNGNGC GGGCCTCTTC GCTATTACGC
              121 CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC
              181 CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAGCGCGCGT AATACGACTC ACTATAGGGC
              241 GAATTGGGTA CTTTCGCTGT CCCCAGTGCA TATTCAGGTA CCGGGCCCCC CCTCGAGGTC
              301 NACNGNATCN NNNNNNNNGA TATCNAATTC CTGCNNNNNN NGGGATCCNC TAGTTCTAGA
              361 GCGGCCGCCA CCGCGGTGGA GCTCCTGAAT ATGCACTGGG GACAGCGAAA GCTCCAGCTT
            421 TTGTTCCCTT TANTGAGGGN NANNNNNNNN NN
            //
            Primer: lacIpro-B_F (Pr0724)
            Region: lac pro,lacO,T3 pro,syn-crRNA-TS_9-12a.2,MCS,syn-crRNA-TS_9-12a.1
            Sequence file: RDB20013_B3Gkb.seq check
            >D09326A1_B3Gk_1_lacIpro-B_F_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNGN NNNNNNTCNG TGNNCGNNGA ANCGGANNAN CGCCCAATAC
               61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC
              121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG
              181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT
              241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC
              301 ACTAAAGGGA ACAAAAGCTG GAGCTTTCGC TGTCCCCAGT GCATATTCAG GAGCTCCACC
              361 GCGGTGGCGG CCGCTCTAGA ACTAGTGGAT CCCCCGGGCT GCAGGAATTC GATATCAAGC
              421 TTATCGATAC CGTCGACCTC GAGGGGGGGC CCGGTACCTG AATATGCACT GGGGACAGCG
              481 AAAGTACNNN NNNCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC CGTCGTTTTA
              541 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC
              601 CCTTTCGCCA GCTGGCGTAA TAGCGAANAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG
              661 CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG
              721 GTGGTTACGC GCANCGTGNN CGCTACACTT GCCAGCGCCC TANCGCCCGC TCCTTTCGCT
              781 TTCTTCCCTT CCTTTCTCGC CACGTNCGCC GGCTTNCCCC GNCNNGCTCT AANTCGGGGG
              841 CTCCCTTTAG GGTTCCGATT TAGNGNTTNN CNNCNNCTCN NNCCCCAAAA AACTTGANTA
            901 NGGGNNNNGG NTCNNGNANN NGGNCNNTNN NNCNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Ishibashi, R., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. [link to RRC of NBRP]

            2023.08.14

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