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pCriMGET_9-12a (#RDB20013)

Plasmid vector to construct a donor cassette for knock-in of long external DNA cleaved by both CRISPR-Cas9 and CRISPR-Cas12a.

Clone info. Plasmid vector to clone a targeting cassette. By using Syn-crRNA-TS-sgRNA with Cas9 nuclease, or Syn-crRNA-TS-crRNA with Cas12a, the targeting cassette can be excised in vivo. See Fig.1 of Ishibashi, R. et al., Sci. Rep., 12(1): 17775, 2022.
Vector backbone pBluescript II SK(+) (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Amp, 30oC
Growth remarks NEB Stable or an equivalent host is recommended for amplifying this clone.
Gene/insert name synthetic sequence (Syn-crRNA-TS_9-12a)
Depositor|Developer Ishibashi, Riki |
 
Sequence (full) RDB20013hts01.seq checkAssembled from experimentally sequenced data.
Publication Ishibashi, R., Sci. Rep. 12 (1): 17775 (2022). [link to RRC of NBRP]
Test sheet RDB20013_B3Ijp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ishibashi, R. et al., Sci. Rep. 12(1): 17775, 2022)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること (Ishibashi, R. et al., Sci. Rep. 12(1): 17775, 2022)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20013 pCriMGET_9-12a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCriMGET_9-12a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20013).

Reference section:

Ishibashi, R., Maki, R., Kitano, S., Miyachi, H., Toyoshima, F., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ishibashi, R., Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a. Sci. Rep. 12 (1): 17775 (2022). PMID 36272994. [link to RRC of NBRP]