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Tyrosinase KO nickase2 vector

Tyrosinase knockout vector which encodes Cas9 nickase and gRNA (2) for tyrosinase.

Catalog number RDB20023
Resource name Tyrosinase KO nickase2 vector
Clone info. Tyrosinase knockout vector which encodes Cas9 nickase and gRNA (2) for tyrosinase. See Fig.3 of Kamo, H. et al., Oncol Lett., 23 (5): 169, 2022.
Vector backbone pSpCas9n(BB)-2A-Puro (PX462) V2.0 (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cells)
Gene/insert name human TYR oligo DNA.
Depositor|Developer Simizu, Siro |
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External Database
human TYR
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Kamo, H. et al. Oncol. Lett. 23(5): 169, 2023)

          Additional terms and conditions:
          The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
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          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用すること(Kamo, H. et al. Oncol. Lett. 23(5): 169, 2023)。
          付加的提供条件：
          このリソースは、非営利機関の非営利学術研究に限って提供する。
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          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB20023 Tyrosinase KO nickase2 vector Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The Tyrosinase KO nickase2 vector was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20023).

          Reference section:

          Kamo, H., Kawahara, R., Simizu, S., Tyrosinase suppresses vasculogenic mimicry in human melanoma cells. Oncol. Lett. (5): 169 (2022). PMID 35496574. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Kamo, H., Tyrosinase suppresses vasculogenic mimicry in human melanoma cells. Oncol. Lett. (5): 169 (2022). PMID 35496574. [link to RRC of NBRP]

          2023.11.06

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