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pcDNA3/Cox8a=mStayGold (#RDB20228)

Expression vector of mStayGold as a fluorescent marker of the inner mitochondrial membrane.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ando, R., Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. A fluorescent marker of the inner mitochondrial membrane. Cytochrome c oxidase subunit 8A (COX8A) was fused to the N-terminus of mStayGold through a Coupler linker: (GGGGS)×3, denoted as ‘=’. See Ando, R. et al. Nat. Methods, 2023. https://doi.org/10.1038/s41592-023-02085-6 for more information.
Vector backbone pcDNA3 (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name Cytaeis uchidae GFP (StayGold) cDNA; mouse Cox8a cDNA.
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., Nat. Methods 21 (4): 648-656 (2024).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., Nat. Methods 21 (4): 648-656 (2024).

Catalog # Resource name Shipping form Fee
RDB20228 pcDNA3/Cox8a=mStayGold DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3/Cox8a=mStayGold was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20228).

Reference section:

Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [PubMed] [Article] [RRC of NBRP]