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pCRtk-EEF1A1-iRFP670-BSD (#RDB20282)

Genome editing tool. Donner vector to tag T2A-tandem-humanized iRFP670-T2A-BSD at human EEF1A1.

Clone info. Genome editing tool. Donner vector to tag T2A-tandem-humanized iRFP670-T2A-BSD at human EEF1A1, C-terminaly. Selection with Zeo & GCV.
Vector backbone pCR-BluntII-TOPO (plasmid)
Growth conditions LB+25 Zeo 37dC
Gene/insert name human EEF1A1 genomic DNA; Thosea asigna virus T2A self-cleaving sequence; Rhodopseudomonas palustris iRFP670; Aspergillus terreus Blastcidin S resistance gene; CMV promoter; HSV thymidine kinase cDNA, poly(A) signal.
Depositor|Developer DNA Bank, | Nakashima, Kenichi |
 
Sequence (full) RDB20282hts01.seq checkAssembled from experimentally sequenced data.
Publication Wakimoto, A., bioRxiv doi: 10.1101/2024.05.09.593461 (2024). [link to Journal]
Test sheet RDB20282C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Arata, A. et al. bioRxiv doi: https://doi.org/10.1101/2024.05.09.593461)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Arata, A. et al. bioRxiv doi: https://doi.org/10.1101/2024.05.09.593461)

Catalog # Resource name Shipping form Fee
RDB20282 pCRtk-EEF1A1-iRFP670-BSD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCRtk-EEF1A1-iRFP670-BSD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20282).

Reference section:

Wakimoto, A., Jeon, H., Shahri, Z. J., Hayashi, T., Liao, C.-W., Gogoleva, N., Suchy, F., Noda, A., An, Y., Nakauchi, H., Hayashi, Y., Hamada, M., Takahashi, S., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). [link to Journal]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Wakimoto, A., Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival. bioRxiv doi: 10.1101/2024.05.09.593461 (2024). [link to Journal]