Resource data sheet
DNA Bank Top

CS2-CMV-nGRAPHIC-P2A-mCherry (#RDB20429)

Expression vector of n-terminal sfGFP on cell membrane with GPI anchor , co-expression of mCherry via P2A peptide.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Clone info. Expression vector of n-terminal sfGFP on cell membrane with GPI anchor , co-expression of mCherry via P2A peptide.
Vector backbone CSII-CMV-MCS (RDB04377) (plasmid)
Selectable markers Ampicillin
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Kuchimaru, Takahiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
In order to receive this biological resource, please request the Exclusive MTA to us.
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Misa M. et al. Nat Commun. 14(1):8031, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Jichi Medical University.
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Misa M. et al. Nat Commun. 14(1):8031, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、自治医科大学の許可を得ること。
付加的使用条件：
レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee
RDB20429 CS2-CMV-nGRAPHIC-P2A-mCherry DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS2-CMV-nGRAPHIC-P2A-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20429).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles