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pCAG-His-iSH-SspB-P2AT2A-mVenus-iLID-CAAX (#RDB20664)

Expression vector of PPAP2.1.

Alternative name pCAG-PPAP2.1
Clone info. Expression vector of PPAP2.1. See Fig.1B of Ueda, Y., Cell Chem. Biol., 29 (11):1576-1587, 2022. e5. doi: 10.1016/j.chembiol.2022.10.002.
Vector backbone pCAG vector (plasmid)
Selectable markers Ampicillin
Gene/insert name Synthetic His-tag; Bos taurus PIK3R1 (inter-SH2 domain); Haemophilus influenzae stringent starvation protein B (SspB); porcine teschovirus self-cleavage sequence (P2A); Thosea asigna self-cleavage sequence (T2A); Aequorea victoria GFP (mVenus); Avena sativa phototropin-1 (LOV2 domain); E. coli SsrA; human KRAS (CAAX motif).
Depositor|Developer Ueda, Yoshibumi |
 
Sequence (full) RDB20664hts01.seq checkAssembled from experimentally sequenced data.
Publication Ueda, Y., Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]
Test sheet RDB20664C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the National Institute of Infectious Diseases.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022) 。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、国立感染症研究所の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20664 pCAG-His-iSH-SspB-P2AT2A-mVenus-iLID-CAAX DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAG-His-iSH-SspB-P2AT2A-mVenus-iLID-CAAX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20664).

Reference section:

Ueda, Y., Miura, Y., Tomishige, N., Sugimoto, N., Murase, M., Kawamura, G., Sasaki, N., Ishiwata, T., Ozawa, T., Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ueda, Y., Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]