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pCAGkawa-His-iSH-SspB-mCherry-P2AT2A-iLID-CAAX (#RDB20665)

Expression vector of PPAP2.2.

Alternative name pCAG-PPAP2.2
Clone info. Expression vector of PPAP2.2. See Fig.1B of Ueda, Y., Cell Chem. Biol., 29 (11):1576-1587, 2022. e5. doi: 10.1016/j.chembiol.2022.10.002.
Vector backbone pCAG vector (plasmid)
Selectable markers Ampicillin
Gene/insert name Synthetic His-tag; Bos taurus PIK3R1 (inter-SH2 domain); Haemophilus influenzae stringent starvation protein B (SspB); Discosoma sp. RFP (mCherry); porcine teschovirus self-cleavage sequence (P2A); Thosea asigna self-cleavage sequence (T2A); Avena sativa phototropin-1 (LOV2 domain); E. coli SsrA; human KRAS (CAAX motif).
Depositor|Developer Ueda, Yoshibumi |
 
Sequence (full) RDB20665hts01.seq checkAssembled from experimentally sequenced data.
Publication Ueda, Y., Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]
Test sheet RDB20665C0p1-1.pdf 
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the National Institute of Infectious Diseases.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022) 。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、国立感染症研究所の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20665 pCAGkawa-His-iSH-SspB-mCherry-P2AT2A-iLID-CAAX DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pCAGkawa-His-iSH-SspB-mCherry-P2AT2A-iLID-CAAX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20665).

Reference section:

Ueda, Y., Miura, Y., Tomishige, N., Sugimoto, N., Murase, M., Kawamura, G., Sasaki, N., Ishiwata, T., Ozawa, T., Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ueda, Y., Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]