Resource data sheet
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pT2K-BI-TRE-HA-Lats2/EGFP (#RDB20709)

Bidirectional expression vector of mouse Lats2 (HA-tag at N-terminus) and EGFP. Tet-inducible. Tol2 transposon vector.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Hirono, N., Development 152 (1): dev203091 (2025). PMID 39629521. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Bidirectional expression vector of mouse Lats2 (HA-tag at N-terminus) and EGFP. Tet-inducible. Tol2 transposon vector. See Fig. 2A of Hirono, N. et al., Development (2025) 152, dev203091. doi:10.1242/dev.203091.
Vector backbone pT2K-BI-TRE-EGFP (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37℃
Gene/insert name mouse Lats2 cDNA.
Depositor|Developer Sasaki, Hiroshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Hirono N. et al., Development, 152, dev203091.2025). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional terms and conditions:
Regarding pT2K-BI-TRE-EGFP: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature of pT2K-BI-TRE-EGFP is requested (Sato, Y. et al. Dev. Biol. 305 (2): 616-624 (2007)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hirono N. et al., Development, 152, dev203091.2025)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。
付加的使用条件：
pT2K-BI-TRE-EGFPについて: 利用者は、研究成果の公表にあたってpT2K-BI-TRE-EGFPの文献を引用する(Sato, Y. et al. Dev. Biol. 305 (2): 616-624 (2007))。

Catalog # Resource name Shipping form Fee
RDB20709 pT2K-BI-TRE-HA-Lats2/EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pT2K-BI-TRE-HA-Lats2/EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20709).

Reference section:

Hirono, N., Hashimoto, M., Shimojo, H., Sasaki, H., Fate specification triggers a positive feedback loop of TEAD-YAP and NANOG to promote epiblast formation in preimplantation embryos. Development 152 (1): dev203091 (2025). PMID 39629521. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirono, N., Fate specification triggers a positive feedback loop of TEAD-YAP and NANOG to promote epiblast formation in preimplantation embryos. Development 152 (1): dev203091 (2025). PMID 39629521. [PubMed] [Article] [RRC of NBRP]