Resource data sheet
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pCAG_SKP1-1D4 (#RDB20821)

Expression vector of mouse SKP1. 1D4-tag at C-terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Kaneda, Y., Elife 13: RP92794 (2024). PMID 39163107. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse SKP1. 1D4-tag at C-terminus. See Fig.1D of Kaneda, Y. et al., Elife, 13:RP92794, 2024. doi: 10.7554/eLife.92794.
Vector backbone pCAG1.1 (RDB19246) (plasmid)
Selectable markers Ampicillin
Growth conditions LB+Ampicillin, 37oC
Gene/insert name mouse Skp1 cDNA.
Depositor|Developer Ikawa, Masahito | Miyata, Haruhiko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Kaneda Y, ert al., Elife, 13:RP92794, 2024, doi: 10.7554/eLife.92794. 2024.10.23) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kaneda Y, ert al., Elife, 13:RP92794, 2024, doi: 10.7554/eLife.92794. 2024.10.23)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee
RDB20821 pCAG_SKP1-1D4 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCAG_SKP1-1D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20821).

Reference section:

Kaneda, Y., Miyata, H., Xu, Z., Shimada, K., Kamoshita, M., Nakagawa, T., Emori, C., Ikawa, M., FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility. Elife 13: RP92794 (2024). PMID 39163107. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Kaneda, Y., FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility. Elife 13: RP92794 (2024). PMID 39163107. [PubMed] [Article] [RRC of NBRP]