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pCDNA3-HygR (#RDB20899)

Mammalian expression vector of hygromicin resistance gene, coding Streptomyces hygroscopicus phosphotransferase.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Dei Cas, M., J. Lipid Res. 65 (3): 100517 (2024). PMID 38342436. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Mammalian expression vector of hygromicin resistance gene, coding Streptomyces hygroscopicus phosphotransferase.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli); Neomycin (mammalian cell)
Growth conditions (e.g. LB+Ampicillin, 37oC )
Gene/insert name Streptomyces hygroscopicus hygromycin B phosphotransferase genomic DNA.
Depositor|Developer Trinchera, Marco |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Dei Cas, M. et al. J. Lipid. Res., 65 (3): 100517, 2024) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the University of Insubria.
提供案内 (日本国内) [open/close]

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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Dei Cas, M. et al. J. Lipid. Res., 65 (3): 100517, 2024) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the University of Insubria.

Catalog # Resource name Shipping form Fee
RDB20899 pCDNA3-HygR DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCDNA3-HygR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20899).

Reference section:

Dei Cas, M., Montavoci, L., Pasini, C., Caretti, A., Penati, S., Martinelli, C., Gianelli, U., Casati, S., Nardecchia, F., Torella, A., Brunetti-Pierri, N., Trinchera, M., Loss of function and reduced levels of sphingolipid desaturase DEGS1 variants are both relevant in disease mechanism. J. Lipid Res. 65 (3): 100517 (2024). PMID 38342436. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Dei Cas, M., Loss of function and reduced levels of sphingolipid desaturase DEGS1 variants are both relevant in disease mechanism. J. Lipid Res. 65 (3): 100517 (2024). PMID 38342436. [PubMed] [Article] [RRC of NBRP]