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Sulfolobus tokodaii Protein Expression Plasmid - StoEx08F05

Catalog Number THR043325
Clone Name StoEx08F05
Vector pET21a (no His-tag)
Plasmid ID of the Whole Cell Project ST0323
Description Bacterial expression plasmid clone of Sulfolobus tokodaii STK_RS01830.
426aa long hypothetical histidyl-tRNA synthetase
Note ST0323
Depositor Kawarabayasi, Yutaka
 
Source Sulfolobus tokodaii strain7
Locus symbol hisS
Synonym -
NCBI locus tag ST0323
Protein Name histidyl-tRNA synthetase
NCBI Gene ID 1458237
Other clones KEGG orthologs (KEGG KO K01892) in our bank
KEGG gene STK_03230
Links

  KEGG gene

  KEGG Ortholog

  NCBI Gene

Distribution information

Ordering information ordering information

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the literature specified by the DEPOSITOR.
 
Catalog # Clone name Shipping form Fee (non-profit org.)
THR043325 StoEx08F05 DNA solution

Status and protocol provided by the depositor.

Expressed Soluble Purified Protocol
yes yes    

This clone has been sequenced a portion for verification.

Seq File Sto08F05a.seq
>StoEx08F05 (THR043325) 5' side
    1 GAATTCCCTG AATATTTTGT TTACTTTAGA AGGAGATATA CCATGGGAAC TGAAGAGACA
   61 ATTAAAAACC CAGACAAAAC AATCGAAAAC GGAGATACTA TTTCCGTTGA TTATGTTGGA
  121 AAACTGGAAG ACGGCACTGT TTTCGACACA TCGGAAAAAG AAGCAGCGAG CGAGGCAGGG
  181 ATATACAACG AAATGAGGGA CTACAAACCT CTGACGTTCA CTGTGGGAGC GGGGGAAATG
  241 ATAAAAGGCT TTGACGAGGG CGTAGTCGGA ATGAAAGTAG GAGAGGAAAA AACTCTTGAA
  301 ATCCCTCCAG AGGAAGCATA CGGAGAATAC ATGGAAGAGT ACGTAAGGGA AATCCCACGA
  361 AACGCAGTCA ATTTTACTCC CGAAACCGGG ATGCAACTTG CTACGGAAAA CGGGCTTCGG
  421 GGAAAGGTCA CTGAAGTAGG CGAGGAAAAC TTCGTAGTGG ACTTCAACCA TGAGCTTGCT
  481 GGAAAGACCC TGATATTCAA AATAAAAGTC ATATCATTGG AGGCAACTAG TCTGGTTCCG
  541 CGTGGATCCC ATATGATGAT TTCTTATGAG CCAGTAAGAG GAATGAAAGA CTATTATGGC
  601 GAAGAGCTTC ATAAAATAAA AGTAGTCGAG AATGCTTTTT TGAAAATTGT AAAACTAGCT
  661 GGATATCAAG AAGTGGAAAC ACCTATTGTA GAAGATTTTC AACTATTCGC TTTAAAAGGA
  721 GGAGAAGAAC TTAGAAATAC AATGTATGTC TTTAAAGATA AGGCCGGTAG AGAAGTTGCA
781 TTAAGACCCG AGTTCACACC TAGTATAGTT AGATTTTATC TTAATTCATT ACAACNTN
//

References and tips

References

reference Kawarabayasi, Y. Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7. DNA Res. 8: 123-140 (2001). PMID 11572479

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2019.04.23

GeneID_ko_thermos_clone_190418.csv - GNP_filter3_thermos_190421.pl