Strain Data Sheet

RBRC00045

Strain Information

Image
BRC No.RBRC00045
TypeSpontaneous Mutation
SpeciesMus musculus
Strain nameB6C3Fe-a/a-Reln<rl>
Former Common name
H-2 Haplotype
ES Cell line
Background strainmixed background of B6 and C3H.
Appearanceblack, unaffected [a/a +/? or a/a Relnrl/+]
black, ataxic [a/a Relnrl/Relnrl]
Strain developmentThis substrain of reeler mice were developed by Ogawa M (Neuron14, 1995) by extensive selection ofhomozygous breeders from original mutant mice. The strain has been maintained by sister brother mating for more than15 generations in RIKEN Tsukuba Institute.
Strain descriptionThe reeler mutation was first identified by Falconer as neurological mutant mice with abnormal locomotion (J Genet 50, 192-201, 1951) . Laminar formation of cerebral and cerebellar cortex was severely impaired in homozygous mutant mice. This mutant lacks entire Relin gene associated with large deletion of about 150kb.
Colony maintenanceHeterozygous females x homozygous males, or heterozygote x heterozygote. Some homozygous females may also be used as breeders.
References
The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons.
Hirotsune S, Takahara T, Sasaki N, Hirose K, Yoshiki A, Ohashi T, Kusakabe M, Murakami Y, Muramatsu M, Watanabe S, et a l
Nat. Genet., 10, 77-83 (1995). 7647795

The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons.
Ogawa M, Miyata T, Nakajima K, Yagyu K, Seike M, Ikenaka K, Yamamoto H, Mikoshiba K
Neuron, 14, 899-912 (1995). 7748558

Reelin controls position of autonomic neurons in the spinal cord.
Yip J W, Yip Y P, Nakajima K, Capriotti C
Proc. Natl. Acad. Sci. USA, 97, 8612-8616 (2000). 10880573

Autosomal recessive lissencephaly with cerebellar hypoplasia is associated with human RELN mutations.
Hong S E, Shugart Y Y, Huang D T, Shahwan S A, Grant P E, Hourihane J O, Martin N D, Walsh C A
Nat. Genet., 26, 93-96 (2000). 10973257

Down-regulation of dendritic spine and glutamic acid decarboxylase 67 expressions in the reelin haploinsufficient heterozygous reeler mouse.
Liu W S, Pesold C, Rodriguez M A, Carboni G, Auta J, Lacor P, Larson J, Condie B G, Guidotti A, Costa E
Proc. Natl. Acad. Sci. USA, 98, 3477-3482 (2001). 11248103

Cortical upper layer neurons derive from the subventricular zone as indicated by Svet1 gene expression.
Tarabykin V, Stoykova A, Usman N, Gruss P
Development, 128, 1983-1993 (2001). 11493521

The reelin pathway modulates the structure and function of retinal synaptic circuitry.
Rice D S, Nusinowitz S, Azimi A M, Martinez A, Soriano E, Curran T
Neuron, 31, 929-941 (2001). 11580894

Health Report

Examination Date / Room / Rack2024/08/26Room:4-BRack:GSentinel mouse program
2024/05/27Room:4-BRack:GSentinel mouse program
2024/02/26Room:4-BRack:GSentinel mouse program
2023/11/27Room:4-BRack:GSentinel mouse program
2023/08/28Room:4-BRack:GSentinel mouse program
2023/05/29Room:4-BRack:GSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
a
MGI:87853		nonagouti2a<a>
MGI:1855937		nonagouti
Reln
MGI:103022		reelin5Reln<rl>
MGI:1856398		reeler
  • epilepsy, familial temporal lobe, 1(MedGEN)

  • familial temporal lobe epilepsy 7(MedGEN)

  • Norman-Roberts syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • Purkinje cell degeneration(MP:0000876)

  • abnormal cerebellum morphology(MP:0000849)

  • abnormal cerebral cortex morphology(MP:0000788)

  • abnormal cortical marginal zone morphology(MP:0000792)

  • abnormal dentate gyrus morphology(MP:0000812)
    • more 37 phenotypes
  • abnormal entorhinal cortex morphology(MP:0006300)

  • abnormal gait(MP:0001406)

  • abnormal hippocampus CA1 region morphology(MP:0008263)

  • abnormal hippocampus CA2 region morphology(MP:0008265)

  • abnormal hippocampus morphology(MP:0000807)

  • abnormal hippocampus pyramidal cell layer(MP:0008284)

  • abnormal neuron differentiation(MP:0009937)

  • abnormal neuronal migration(MP:0006009)

  • abnormal olfactory cortex morphology(MP:0005267)

  • abnormal prepulse inhibition(MP:0003088)

  • abnormal sensory neuron innervation pattern(MP:0000968)

  • abnormal sexual interaction(MP:0002566)

  • abnormal short-term spatial reference memory(MP:0008431)

  • abnormal social investigation(MP:0001360)

  • abnormal stratification in cerebral cortex(MP:0000790)

  • abnormal ventral striatum morphology(MP:0004103)

  • ataxia(MP:0001393)

  • behavior/neurological phenotype(MP:0005386)

  • decreased Purkinje cell number(MP:0000880)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased dopaminergic neuron number(MP:0011448)

  • decreased locomotor activity(MP:0001402)

  • diarrhea(MP:0005036)

  • disheveled coat(MP:0001511)

  • impaired balance(MP:0001525)

  • impaired righting response(MP:0001523)

  • infertility(MP:0001924)

  • lethality at weaning, complete penetrance(MP:0011083)

  • male infertility(MP:0001925)

  • nervous system phenotype(MP:0003631)

  • postnatal growth retardation(MP:0001732)

  • premature death(MP:0002083)

  • reduced female fertility(MP:0001923)

  • slow postnatal weight gain(MP:0008489)

  • small cerebellum(MP:0000852)

  • tremors(MP:0000745)
    • Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationDevelopmental Biology Research
    Mouse Models for Human Disease
    Neurobiology Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorMasaharu Ogawa (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data