Strain Data Sheet

RBRC00158

Strain Information

Image
BRC No.RBRC00158
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tg(gpt delta)1Nmi/Rbrc
Former Common nameC57BL/6-TgN(gpt Delta)
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a]
Strain developmentThis strain was developed for efficient detection of mutations in vivo by Dr.Nohmi T.
Strain descriptionThis strain is useful to detect point mutations and deletions with high efficiency and sensitivity in vivo. The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing chloramphenicol and 6-thioguanine. The EG10 DNA carries a chi site along with the red and gam genes so that the wild-type phages display Spi-(sensitive to P2 interference) phenotype. Mutant phages lacking both red and gam genes can be positively detected as plaques that grow in P2 lysogens of E. coli.
Colony maintenance
References
A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections.
Nohmi T, Katoh M, Suzuki H, Matsui M, Yamada M, Watanabe M, Suzuki M, Horiya N, Ueda O, Shibuya T, Ikeda H, Sofuni T
Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079

Spectra of gpt mutations in ethylnitrosourea-treated and untreated transgenic mice.
Masumura K, Matsui M, Katoh M, Horiya N, Ueda O, Tanabe H, Yamada M, Suzuki H, Sofuni T, Nohmi T
Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717

Spi(-) selection: An efficient method to detect gamma-ray-induced deletions in transgenic mice.
Nohmi T, Suzuki M, Masumura K, Yamada M, Matsui K, Ueda O, Suzuki H, Katoh M, Ikeda H, Sofuni T
Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718

Mutagenicity of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) in the new gpt delta transgenic mouse.
Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Ueda O, Suzuki H, Kanke Y, Tindall K R, Wakabayashi K, Sofuni T, Nohmi T
Cancer Lett., 143, 241-244 (1999). 10503911

UVB-induced gpt mutations in the skin of gpt delta transgenic mice.
Horiguchi M, Masumura K, Ikehata H, Ono T, Kanke Y, Sofuni T, Nohmi T
Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728

Characterization of mutations induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in the colon of gpt delta transgenic mouse: novel G:C deletions beside runs of identical bases.
Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Wakabayashi K, Nohmi T
Carcinogenesis, 21, 2049-2056 (2000). 11062167

Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
Minowa O, Arai T, Hirano M, Monden Y, Nakai S, Fukuda M, Itoh M, Takano H, Hippou Y, Aburatani H, Masumura K, Nohmi T, Nishimura S, Noda T
Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358

Environ. Mutagen Res., 22, 85-90 (2000).

Molecular nature of ultraviolet B light-induced deletions in the murine epidermis.
Horiguchi M, Masumura K I, Ikehata H, Ono T, Kanke Y, Nohmi T
Cancer Res., 61, 3913-3918 (2001). 11358805

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
cat E. coli chloramphenicol acetyltransferase (CAT)17
gpt Delta guanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>
MGI:3612991		transgene insertion 1, Takehito Nohmi
loxP phage P1 loxP17loxP
loxP phage P1 loxP17loxP
red/gam lambda phage red/gam17red/gam

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal cell physiology(MP:0005621)
    • Detailed phenotype data

    Ordering Information

    Donor DNAlambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
    Research application
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorTakehiko Noumi (National Institute of Health Sciences)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data