Strain Data Sheet

RBRC00159

Strain Information

Image
BRC No.RBRC00159
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Trp53<tm1Sia> Tg(gpt delta)1Nmi/Rbrc
Former Common nameC57BL/6-TgH(p53)-TgN(gpt delta), C57BL/6-p53 <tm1Sia>-Tg(gpt Delta)
H-2 Haplotype
ES Cell line
Background strainC57BL/6
Appearanceblack [a/a]
Strain developmentThe original p53 deficient mice had been introduced into C57BL/6 background for 13 generations by Dr. Niwa T at Hiroshima Univ (-1995). Then the mice were transfered to Dr. Kusakabe M. at RIKEN Life Science Center for further backcrossing to establish the B6 congenic strain. The gpt delta mice were crossed with C57BL/6-p53tm1 to establish a double mutant at RIKEN, and have been maintained by sister-brother mating for 8 generations.
Strain description
Colony maintenance
References
Enhanced proliferative potential in culture of cells from p53-deficient mice.
Tsukada T, Tomooka Y, Takai S, Ueda Y, Nishikawa S, Yagi T, Tokunaga T, Takeda N, Suda Y, Abe S, et a l
Oncogene, 8, 3313-3322 (1993). 7504233

A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections.
Nohmi T, Katoh M, Suzuki H, Matsui M, Yamada M, Watanabe M, Suzuki M, Horiya N, Ueda O, Shibuya T, Ikeda H, Sofuni T
Environ. Mol. Mutagen., 28, 465-470 (1996). 8991079

UVB-induced gpt mutations in the skin of gpt delta transgenic mice.
Horiguchi M, Masumura K, Ikehata H, Ono T, Kanke Y, Sofuni T, Nohmi T
Environ. Mol. Mutagen., 34, 72-79 (1999). 10529728

Spectra of gpt mutations in ethylnitrosourea-treated and untreated transgenic mice.
Masumura K, Matsui M, Katoh M, Horiya N, Ueda O, Tanabe H, Yamada M, Suzuki H, Sofuni T, Nohmi T
Environ. Mol. Mutagen., 34, 1-8 (1999). 10462717

Mutagenicity of 2-amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP) in the new gpt delta transgenic mouse.
Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Ueda O, Suzuki H, Kanke Y, Tindall K R, Wakabayashi K, Sofuni T, Nohmi T
Cancer Lett., 143, 241-244 (1999). 10503911

Spi(-) selection: An efficient method to detect gamma-ray-induced deletions in transgenic mice.
Nohmi T, Suzuki M, Masumura K, Yamada M, Matsui K, Ueda O, Suzuki H, Katoh M, Ikeda H, Sofuni T
Environ. Mol. Mutagen., 34, 9-15 (1999). 10462718

Characterization of mutations induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in the colon of gpt delta transgenic mouse: novel G:C deletions beside runs of identical bases.
Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Wakabayashi K, Nohmi T
Carcinogenesis, 21, 2049-2056 (2000). 11062167

Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
Minowa O, Arai T, Hirano M, Monden Y, Nakai S, Fukuda M, Itoh M, Takano H, Hippou Y, Aburatani H, Masumura K, Nohmi T, Nishimura S, Noda T
Proc. Natl. Acad. Sci. USA, 97, 4156-4161 (2000). 10725358

Environ. Mutagen Res., 22, 85-90 (2000).

Molecular nature of ultraviolet B light-induced deletions in the murine epidermis.
Horiguchi M, Masumura K I, Ikehata H, Ono T, Kanke Y, Nohmi T
Cancer Res., 61, 3913-3918 (2001). 11358805
more 1 references
Mutation induction by heavy-ion irradiation of gpt delta transgenic mice.
Yatagai F, Nohmi T, Kusakabe M, Masumura K, Yoshiki A, Yamaguchi H, Kurobe T, Kuniya K, Hanaoka F, Yano Y
Phys. Med. 17 Suppl. 1:192-193, (2001). 11776258

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Trp53
MGI:98834		transformation related protein 5311Trp53<tm1Sia>
MGI:1926340		targeted mutation 1, Shinichi Aizawa
  • adrenal cortex carcinoma(DisGeNET)

  • adrenocortical carcinoma, hereditary(MedGEN)

  • B-cell chronic lymphocytic leukemia(DisGeNET)
    • more 14 Diseases
  • basal cell carcinoma, susceptibility to, 7(MedGEN)

  • bone marrow failure syndrome 5(MedGEN)

  • bone osteosarcoma(MedGEN)

  • breast carcinoma(DisGeNET)

  • choroid plexus papilloma(MedGEN)

  • colorectal cancer(MedGEN)

  • exocrine pancreatic carcinoma(MedGEN)

  • glioma susceptibility 1(MedGEN)

  • glioma(DisGeNET)

  • hepatocellular carcinoma(MedGEN)

  • hereditary breast carcinoma(MedGEN)

  • Li-Fraumeni syndrome(DisGeNET)

  • nasopharyngeal carcinoma(MedGEN)

  • obsolete Li-Fraumeni syndrome 1(MedGEN)
    • neo neomycin resistance gene (E. coli)11 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    cat E. coli chloramphenicol acetyltransferase (CAT)17
    gpt Delta guanine phosphoribosyltransferase17gpt Delta<Tg(gptdelta)1Nmi>
    MGI:3612991    		transgene insertion 1, Takehito Nohmi
    loxP phage P1 loxP17loxP
    loxP phage P1 loxP17loxP
    red/gam lambda phage red/gam17red/gam

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal cell proliferation(MP:0000350)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • increased T cell derived lymphoma incidence(MP:0002024)

  • increased sensitivity to induced cell death(MP:0008943)

  • increased tumor incidence(MP:0002020)
    • more 1 phenotypes
  • perinatal lethality, incomplete penetrance(MP:0011090)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, HSV tk polyA, mouse p53 genomic DNA, lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC
    Research applicationApoptosis Research
    Cancer Research
    Cell Biology Research
    Immunology and Inflammation Research
    Mouse Models for Human Disease
    Toxicology Research
    Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
    DepositorRIKEN BRC (RIKEN BRC)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    Nishikawa A, Imazawa T, Kuroiwa Y, Kitamura Y, Kanki K, Ishii Y, Umemura T, Hirose M.
    Induction of colon tumors in C57BL/6J mice fed MeIQx, IQ, or PhIP followed by dextran sulfate sodium treatment.
    Toxicol Sci 84(2) 243-8(2005) 15635146
    Sakai H, Tsukamoto T, Yamamoto M, Shirai N, Iidaka T, Hirata A, Yanai T, Masegi T, Donehower LA, Tatematsu M.
    High susceptibility of nullizygous p53 knockout mice to colorectal tumor induction by 1,2-dimethylhydrazine.
    J Cancer Res Clin Oncol 129(6) 335-40(2003) 12743811
    Masumura K, Horiguchi M, Nishikawa A, Umemura T, Kanki K, Kanke Y, Nohmi T.
    Low dose genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) in gpt delta transgenic mice.
    Mutat Res 541(1-2) 91-102(2003) 14568298