Strain Data Sheet

RBRC00398

Strain Information

Image
BRC No.RBRC00398
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Mmp2<tm1Ito>/ItoRbrc
Former Common nameGelA KO (Nov. 2011), B6.129P2-Mmp2tm1Ito
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Takeshi Itoh and Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1997. The mutant mice were backcrossed to C57BL/6.
Strain descriptionThis strain was generated by gene targeting of gelatinase A (Mmp2, metalloproteinase 2). Homozygous mutant mice are completely devoid of gelatinase A. 5.9 kb of the gene including exon 1 was replaced with a PGK-neo cassette. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
Colony maintenanceHomozygote x Heterozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile, but show a subtle delay in their growth.
References
Unaltered secretion of beta-amyloid precursor protein in gelatinase A (matrix metalloproteinase 2)-deficient mice.
Itoh T, Ikeda T, Gomi H, Nakao S, Suzuki T, Itohara S
J. Biol. Chem., 272, 22389-22392 (1997). 9278386

Health Report

Examination Date / Room / Rack2025/01/07Room:3-ARack:BSentinel mouse program
2024/10/07Room:3-ARack:BSentinel mouse program
2024/07/08Room:3-ARack:BSentinel mouse program
2024/04/08Room:3-ARack:BSentinel mouse program
2024/01/09Room:3-ARack:BSentinel mouse program
2023/10/10Room:3-ARack:BSentinel mouse program
2023/07/10Room:3-ARack:BSentinel mouse program
2023/04/10Room:3-ARack:BSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Mmp2
MGI:97009		matrix metallopeptidase 28Mmp2<tm1Ito>
MGI:2386252		targeted mutation 1, Shigeyoshi Itohara
  • multicentric osteolysis, nodulosis, and arthropathy(MedGEN)
    • neo neomycin resistance gene (E. coli)8 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal angiogenesis(MP:0000260)

  • abnormal articular cartilage morphology(MP:0006433)

  • abnormal bone marrow cell physiology(MP:0009278)

  • abnormal cardiovascular system physiology(MP:0001544)

  • abnormal compact bone morphology(MP:0003797)
    • more 35 phenotypes
  • abnormal coronal suture morphology(MP:0003840)

  • abnormal cranium morphology(MP:0000438)

  • abnormal induced retina neovascularization(MP:0009660)

  • abnormal inflammatory response(MP:0001845)

  • abnormal lambdoid suture morphology(MP:0003841)

  • abnormal response to cardiac infarction(MP:0000343)

  • abnormal sagittal suture morphology(MP:0003843)

  • abnormal skeleton development(MP:0002113)

  • abnormal trabecular bone morphology(MP:0000130)

  • abnormal vascular smooth muscle physiology(MP:0005595)

  • arthritis(MP:0002993)

  • brain inflammation(MP:0001847)

  • broad snout(MP:0030189)

  • coronal suture sclerosis(MP:0030360)

  • decreased angiogenesis(MP:0005602)

  • decreased body size(MP:0001265)

  • decreased body weight(MP:0001262)

  • decreased bone mineral density(MP:0000063)

  • decreased bone volume(MP:0010876)

  • decreased inflammatory response(MP:0001876)

  • decreased length of long bones(MP:0004686)

  • decreased osteoblast cell number(MP:0004989)

  • decreased osteoclast cell number(MP:0004985)

  • decreased tumor growth/size(MP:0003447)

  • dilated heart left ventricle(MP:0002753)

  • impaired lung alveolus development(MP:0006027)

  • increased inner canthal distance(MP:0030166)

  • increased susceptibility to experimental autoimmune encephalomyelitis(MP:0004799)

  • lambdoid suture sclerosis(MP:0030361)

  • neoplasm(MP:0002006)

  • postnatal growth retardation(MP:0001732)

  • sagittal suture sclerosis(MP:0030362)

  • short mandible(MP:0000088)

  • short maxilla(MP:0000097)

  • short snout(MP:0000445)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse GelA genomic DNA
    Research applicationCardiovascular Research
    Developmental Biology Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 272, 22389-22392 (1997).
    DepositorShigeyoshi Itohara (RIKEN)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Bormann T, Maus R, Stolper J, Tort Tarrés M, Brandenberger C, Wedekind D, Jonigk D, Welte T, Gauldie J, Kolb M, Maus UA.
    Role of matrix metalloprotease-2 and MMP-9 in experimental lung fibrosis in mice.
    Respir Res 23(1) 180(2022) 35804363
    Huber VJ, Igarashi H, Ueki S, Terumitsu-Tsujita M, Nito C, Ohno K, Suzuki Y, Itoh K, Kwee IL, Nakada T.
    Visualizing the Distribution of Matrix Metalloproteinases in Ischemic Brain Using In Vivo 19F-Magnetic Resonance Spectroscopic Imaging.
    Contrast Media Mol Imaging 2019 8908943(2019) 30723388
    Taylor SH, Yeung CY, Kalson NS, Lu Y, Zigrino P, Starborg T, Warwood S, Holmes DF, Canty-Laird EG, Mauch C, Kadler KE.
    Matrix metalloproteinase 14 is required for fibrous tissue expansion.
    Elife 4 e09345(2015) 26390284
    Cinq-Frais C, Coatrieux C, Savary A, D'Angelo R, Bernis C, Salvayre R, Nègre-Salvayre A, Augé N.
    Annexin II-dependent actin remodelling evoked by hydrogen peroxide requires the metalloproteinase/sphingolipid pathway.
    Redox Biol 4 169-79(2015) 25574848
    Takamiya Y, Fukami K, Yamagishi S, Kaida Y, Nakayama Y, Obara N, Iwatani R, Ando R, Koike K, Matsui T, Nishino Y, Ueda S, Cooper ME, Okuda S.
    Experimental diabetic nephropathy is accelerated in matrix metalloproteinase-2 knockout mice.
    Nephrol Dial Transplant 28(1) 55-62(2013) 23028104
    Hayashi K, Sasamura H, Ishiguro K, Sakamaki Y, Azegami T, Itoh H.
    Regression of glomerulosclerosis in response to transient treatment with angiotensin II blockers is attenuated by blockade of matrix metalloproteinase-2.
    Kidney Int 78(1) 69-78(2010) 20375993
    Motohashi N, Uezumi A, Yada E, Fukada S, Fukushima K, Imaizumi K, Miyagoe-Suzuki Y, Takeda S.
    Muscle CD31(-) CD45(-) side population cells promote muscle regeneration by stimulating proliferation and migration of myoblasts.
    Am J Pathol 173(3) 781-91(2008) 18669618
    Agrawal S, Anderson P, Durbeej M, van Rooijen N, Ivars F, Opdenakker G, Sorokin LM.
    Dystroglycan is selectively cleaved at the parenchymal basement membrane at sites of leukocyte extravasation in experimental autoimmune encephalomyelitis.
    J Exp Med 203(4) 1007-19(2006) 16585265
    Matsusaka H, Ikeuchi M, Matsushima S, Ide T, Kubota T, Feldman AM, Takeshita A, Sunagawa K, Tsutsui H.
    Selective disruption of MMP-2 gene exacerbates myocardial inflammation and dysfunction in mice with cytokine-induced cardiomyopathy.
    Am J Physiol Heart Circ Physiol 289(5) H1858-64(2005) 15937097
    Hayashidani S, Tsutsui H, Ikeuchi M, Shiomi T, Matsusaka H, Kubota T, Imanaka-Yoshida K, Itoh T, Takeshita A.
    Targeted deletion of MMP-2 attenuates early LV rupture and late remodeling after experimental myocardial infarction.
    Am J Physiol Heart Circ Physiol 285(3) H1229-35(2003) 12775562