Strain Data Sheet

RBRC00522

Strain Information

Image
BRC No.RBRC00522
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;129-Rag2<tm1Fwa> Il2rb<tm1Mak>/Rbrc
Former Common nameC57BL/6-Rag2<tm1Fwa>-IL2rb<tm1Och>
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack
Strain development1998 by Koyasu at Keio Univ. School of Med.Original :Rag2tm1Fwa :1992 by F. W. Alt at Columbia Univ., N.Y. USAIL2rbtm1Och:1995 by T. W. Mak at Ontario Cancer Institute, Toronto, Canada
Strain descriptionThey lack T, B and NK cells. Furthermore, Macrophages and DCs have some functional defects.
Colony maintenanceNothing special. Breed well.
References
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, et a l
Cell, 68 (5) 855-67 (1992). 1547487

Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta.
Suzuki H, Kundig T M, Furlonger C, Wakeham A, Timms E, Matsuyama T, Schmits R, Simard J J, Ohashi P S, Griesser H, et a l
Science, 268, 1472-1476 (1995). 7770771

Development of CD4-CD8- alpha beta TCR+NK1.1+ T lymphocytes: thymic selection by self antigen.
Schulz R J, Parkes A, Mizoguchi E, Bhan A K, Koyasu S
J. Immunol., 157, 4379-4389 (1996). 8906813

Critical role of NK but not NKT cells in acute rejection of parental bone marrow cells in F1 hybrid mice.
Suzue K, Reinherz E L, Koyasu S
Eur. J. Immunol., 31, 3147-3152 (2001). 11745331

Critical role of IL-15-IL-15R for antigen-presenting cell functions in the innate immune response.
Ohteki T, Suzue K, Maki C, Ota T, Koyasu S
Nat. Immunol., 2, 1138-1143 (2001). 11702064

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Il2rb
MGI:96550		interleukin 2 receptor, beta chain15Il2rb<tm1Mak>
MGI:1857193		targeted mutation 1, Tak W Mak
  • immunodeficiency 63 with lymphoproliferation and autoimmunity(MedGEN)
    • neo neomycin resistance gene (E. coli)15 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    Rag2
    MGI:97849    		recombination activating gene 22Rag2<tm1Fwa>
    MGI:1858556    		targeted mutation 1, Frederick W Alt
  • combined immunodeficiency with skin granulomas(MedGEN)

  • Omenn syndrome(DisGeNET, MedGEN)

  • severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-negative, NK cell-positive(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell activation(MP:0008217)

  • abnormal B cell differentiation(MP:0002144)

  • abnormal B cell morphology(MP:0004939)

  • abnormal B cell number(MP:0002458)

  • abnormal CD4-positive T cell differentiation(MP:0008076)
    • more 82 phenotypes
  • abnormal CD4-positive, alpha-beta T cell physiology(MP:0005463)

  • abnormal T cell activation(MP:0001828)

  • abnormal T cell clonal deletion(MP:0005002)

  • abnormal T cell differentiation(MP:0002145)

  • abnormal T cell number(MP:0006387)

  • abnormal T cell physiology(MP:0002444)

  • abnormal T cell receptor V(D)J recombination(MP:0008754)

  • abnormal T-helper 1 physiology(MP:0005465)

  • abnormal chemokine secretion(MP:0008722)

  • abnormal common myeloid progenitor cell morphology(MP:0006410)

  • abnormal effector T cell morphology(MP:0002435)

  • abnormal gamma-delta T cell differentiation(MP:0008356)

  • abnormal hair texture(MP:0002074)

  • abnormal immune system organ morphology(MP:0002722)

  • abnormal immune system physiology(MP:0001790)

  • abnormal immunoglobulin V(D)J recombination(MP:0008755)

  • abnormal interferon secretion(MP:0008555)

  • abnormal intestinal epithelium morphology(MP:0000488)

  • abnormal intestinal mucosa morphology(MP:0000511)

  • abnormal locomotor behavior(MP:0001392)

  • abnormal lymph node morphology(MP:0002339)

  • abnormal lymphocyte morphology(MP:0002619)

  • abnormal macrophage physiology(MP:0002451)

  • abnormal marginal zone B cell morphology(MP:0008180)

  • abnormal mononuclear cell morphology(MP:0008247)

  • abnormal response to transplant(MP:0005671)

  • abnormal sex gland morphology(MP:0000653)

  • abnormal spleen morphology(MP:0000689)

  • abnormal spleen periarteriolar lymphoid sheath morphology(MP:0002358)

  • abnormal stomach mucosa morphology(MP:0010792)

  • abnormal thymus morphology(MP:0000703)

  • absent mature B cells(MP:0008212)

  • arrested B cell differentiation(MP:0001802)

  • arrested T cell differentiation(MP:0001825)

  • colitis(MP:0002816)

  • decreased B cell number(MP:0005017)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)

  • decreased CD8-positive, alpha-beta T cell number(MP:0008079)

  • decreased IgM level(MP:0001806)

  • decreased NK cell number(MP:0008045)

  • decreased T cell proliferation(MP:0005095)

  • decreased body size(MP:0001265)

  • decreased double-negative T cell number(MP:0005089)

  • decreased double-positive T cell number(MP:0005092)

  • decreased immature B cell number(MP:0008215)

  • decreased mature B cell number(MP:0008211)

  • decreased memory T cell number(MP:0008050)

  • decreased pre-B cell number(MP:0008209)

  • decreased regulatory T cell number(MP:0004974)

  • decreased susceptibility to bacterial infection induced morbidity/mortality(MP:0009789)

  • decreased susceptibility to parasitic infection(MP:0005026)

  • decreased susceptibility to type IV hypersensitivity reaction(MP:0005616)

  • decreased thymocyte number(MP:0000715)

  • enlarged lymph nodes(MP:0000702)

  • enlarged spleen(MP:0000691)

  • increased CD4-positive, alpha-beta T cell number(MP:0008074)

  • increased CD8-positive, alpha-beta T cell number(MP:0008078)

  • increased IgE level(MP:0002497)

  • increased IgG1 level(MP:0008499)

  • increased activated T cell number(MP:0001829)

  • increased anti-double stranded DNA antibody level(MP:0004762)

  • increased anti-nuclear antigen antibody level(MP:0004794)

  • increased double-negative T cell number(MP:0005090)

  • increased erythroid progenitor cell number(MP:0003135)

  • increased granulocyte number(MP:0000322)

  • increased immature B cell number(MP:0008214)

  • increased macrophage cell number(MP:0005425)

  • increased natural killer cell mediated cytotoxicity(MP:0003080)

  • increased neutrophil cell number(MP:0000219)

  • increased plasma cell number(MP:0008097)

  • increased susceptibility to autoimmune hemolytic anemia(MP:0004827)

  • increased susceptibility to bacterial infection(MP:0002412)

  • increased susceptibility to infection(MP:0002406)

  • liver inflammation(MP:0001860)

  • lung inflammation(MP:0001861)

  • lymph node hypoplasia(MP:0008101)

  • premature death(MP:0002083)

  • small lymph nodes(MP:0002217)

  • small spleen(MP:0000692)

  • spleen hypoplasia(MP:0000694)

  • thymus hypoplasia(MP:0001823)

  • weight loss(MP:0001263)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E.coli neo, herpes simplex virus thymidine kinase polyA additional signal, mouse Rag2 genomic DNA, mouse IL2rb genomic DNA
    Research applicationCell Biology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
    DepositorShigeo Koyasu (Keio University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data