Strain Data Sheet

RBRC00695

Strain Information

Image
BRC No.RBRC00695
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(KRT14-HGF)43Takk/Rbrc
Former Common namehk14-HGF (human cytokeratin14 promoter + human hepatocyte growth factor)
H-2 Haplotype
ES Cell line
Background strain
Appearanceblack [a/a B/B C/C +/+]
black with an aberrant pigmentation in the skin [a/a B/B C/C Tg/?]
Strain developmentDeveloped by Dr. Takahiro Kunisada at Tottori University in 2000. The human HGF cDNA fragment under the contorl of human keratin 14 promoter was injected into the pronuclei of B6D2F1 fertilized eggs.
Strain descriptionThese transgenic mice develop prominent epidermal pigmentation by overexpression of the human HGF gene controlled by the human keratin 14 promoter. The phenotypes of these transgenic mice conform with that of human dermal melanocytosis. This strain is a useful animal model for dermal melanocytosis.
Colony maintenanceSibling Mating (Hemizygote x wild-type, Hemizygote)
References
Keratinocyte expression of transgenic hepatocyte growth factor affects melanocyte development, leading to dermal melanocytosis.
Kunisada T, Yamazaki H, Hirobe T, Kamei S, Omoteno M, Tagaya H, Hemmi H, Koshimizu U, Nakamura T, Hayashi S I
Mech. Dev., 94, 67-78 (2000). 10842060

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HGF hepatocyte growth factor (human)UNHGF<Tg(KRT14-HGF)43Takk>
bkank		transgene insertion 43, Takahiro Kunisadahuman cytokeratin14 promoter

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal dermal pigmentation(MP:0009385)

  • abnormal skin pigmentation(MP:0002095)

  • increased melanocyte number(MP:0012022)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman cytokeratin14 promoter, rabbit beta globin intron, human hepatocyte growth factor cDNA, human cytokeratin14 poly A human cytokeratin14 poly A
    Research application
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTakahiro Kunisada (Gifu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data