Strain Data Sheet

RBRC00696

Strain Information

Image
BRC No.RBRC00696
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(KRT14-Edn3)1Takk/TakkRbrc
Former Common namehk14-ET3 (human cytokeratin14 promoter + mouse endothelin 3 cDNA) B6
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearanceblack with an aberrant pigmentation in the skin [a/a B/B C/C Tg/?]
black [a/a B/B C/C +/+]
Strain developmentDeveloped by Dr. Takahiro Kunisada at Gifu University school of medicine in 1998. The mouse ET3 cDNA fragment under the human keratin 14 promoter was injected into the pronuclei of B6D2F1 fertilized eggs. The transgenic mice were maintained by brother-sister mating after backcrossing to C57BL/6Cr mice for 20 generations.
Strain descriptionThese transgenic mice develop prominent epidermal pigmentation by overexpression of the mouse endothelin 3 gene controlled by the human keratin 14 promoter. The transgenic mice display a significant increase of the number of melanocytes, and increase thyroid tumorigenesis around 6 months old.
Colony maintenanceSibling Mating (Hemizygote x Hemizygote, wild-type)About 10% of Tg females has small body, and are hard to get pregnant.
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Edn3
MGI:95285		endothelin 3UNEdn3<Tg(KRT14-Edn3)1Takk>
MGI:3687548		transgene insertion 1, Takahiro Kunisadahuman cytokeratin14 promoter
  • Hirschsprung disease, susceptibility to, 4(MedGEN)

  • Waardenburg syndrome type 4B(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal coat/hair pigmentation(MP:0002075)

  • abnormal ear pigmentation(MP:0000015)

  • abnormal melanocyte morphology(MP:0002877)

  • abnormal skin pigmentation(MP:0002095)

  • darkened coat color(MP:0005409)
    • more 4 phenotypes
  • decreased body size(MP:0001265)

  • decreased fertilization frequency(MP:0009647)

  • increased thyroid tumor incidence(MP:0010316)

  • white spotting(MP:0002938)
    • Detailed phenotype data

    Ordering Information

    Donor DNAhuman cytokeratin14 promoter, mouse endothelin 3 cDNA, rabbit beta globin intron, human cytokeratin14 polyA signal
    Research applicationDermatology Research
    Immunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using these mice for 2 year after provided to the RIKEN BRC.
    DepositorTakahiro Kunisada (Gifu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data