Strain Data Sheet

RBRC00753

Strain Information

Image
BRC No.RBRC00753
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Sema4d<tm1Kik>/Rbrc
Former Common nameCD100 Knockout mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
The class IV semaphorin CD100 plays nonredundant roles in the immune system: defective B and T cell activation in CD100-deficient mice.
Shi W, Kumanogoh A, Watanabe C, Uchida J, Wang X, Yasui T, Yukawa K, Ikawa M, Okabe M, Parnes J R, Yoshida K, Kikutani H
Immunity, 13, 633-642 (2000). 11114376

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Sema4d
MGI:109244		sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D13Sema4d<tm1Kik>
MGI:3027762		targeted mutation 1, Hitoshi Kikutani
neo neomycin resistance gene (E. coli)13 herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B-1 B cell morphology(MP:0004940)

  • abnormal immunoglobulin level(MP:0002490)

  • behavior/neurological phenotype(MP:0005386)

  • cardiovascular system phenotype(MP:0005385)

  • decreased B cell proliferation(MP:0005093)
    • more 7 phenotypes
  • decreased B-1a cell number(MP:0008168)

  • decreased CD4-positive, alpha-beta T cell number(MP:0008075)

  • decreased T cell number(MP:0005018)

  • decreased spleen germinal center size(MP:0008484)

  • increased B cell number(MP:0005014)

  • nervous system phenotype(MP:0003631)

  • renal/urinary system phenotype(MP:0005367)
    • Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse CD100 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Immunity, 13, 633-642 (2000).
    DepositorHitoshi Kikutani (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Xiao C, Luo Y, Zhang C, Zhu Z, Yang L, Qiao H, Fu M, Wang G, Yao X, Li W.
    Negative regulation of dendritic cell activation in psoriasis mediated via CD100-plexin-B2.
    J Pathol 250(4) 409-419(2020) 31943215
    Zhang C, Qiao H, Guo W, Liu Y, Yang L, Liu Y, Jin B, Fu M, Wang G, Li W.
    CD100-plexin-B1 induces epithelial-mesenchymal transition of head and neck squamous cell carcinoma and promotes metastasis.
    Cancer Lett 455 1-13(2019) 30981760
    Zhu Z, Luo Y, Yu J, Gao J, Zhang Y, Xiao C, Zhang C, Wang G, Liu Y, Fu M, Yao X, Li W.
    Sema4D is required in both the adaptive and innate immune responses of contact hypersensitivity.
    Mol Immunol 78 98-104(2016) 27614265