Strain Data Sheet

RBRC00754

Strain Information

Image
BRC No.RBRC00754
TypeTargeted Mutation and TransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cd40<tm1Kik> Tg(Igh-CD40)1Kik/Rbrc
Former Common nameCD40 transgenic mice
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeveloped by Hitoshi Kikutani, Research Institute for Microbial Diseases, Osaka University in 2002.
Strain descriptionTransgenic mice expressing wild-type human CD40 gene in a mouse CD40 deficient background. CD40/Tnfrsf5 (tumor necrosis factor receptor superfamily, member 5) is essential for T cell-dependent immunoglobulin class switching and germinal center formation.
Colony maintenance
References
Functional expression of a microinjected Ed alpha gene in C57BL/6 transgenic mice.
Yamamura K, Kikutani H, Folsom V, Clayton L K, Kimoto M, Akira S, Kashiwamura S, Tonegawa S, Kishimoto T
Nature, 316, 67-69 (1985). 2409451

Dissection of B cell differentiation during primary immune responses in mice with altered CD40 signals.
Yasui T, Muraoka M, Takaoka-Shichijo Y, Ishida I, Takegahara N, Uchida J, Kumanogoh A, Suematsu S, Suzuki M, Kikutani H
Int. Immunol., 14, 319-329 (2002). 11867568

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Cd40
MGI:88336		CD40 antigen2Cd40<tm1Kik>
MGI:1857457		targeted mutation 1, Hitoshi Kikutani
  • hyper-IgM syndrome type 3(MedGEN)
    • neo neomycin resistance gene (E. coli)2 herpes simplex virus thymidine kinase promoter (HSV tk promoter)
    CD40 CD40 antigen (human)UNCD40
    MGI:3687555    		mouse E m, IgH promoter, rabbit beta-globin poly A

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal dendritic cell physiology(MP:0002376)

  • abnormal humoral immune response(MP:0001800)

  • absent germinal center B cells(MP:0008179)

  • absent spleen germinal center(MP:0008474)

  • decreased B cell number(MP:0005017)
    • more 17 phenotypes
  • decreased IgA level(MP:0001807)

  • decreased IgE level(MP:0002492)

  • decreased IgG1 level(MP:0008495)

  • decreased IgG2a level(MP:0008496)

  • decreased IgG2b level(MP:0008497)

  • decreased IgG3 level(MP:0008498)

  • decreased bone mineral density(MP:0000063)

  • decreased cerebral infarct size(MP:0006058)

  • decreased compact bone mass(MP:0010962)

  • decreased immunoglobulin level(MP:0002460)

  • decreased neutrophil cell number(MP:0000222)

  • decreased platelet aggregation(MP:0009549)

  • decreased susceptibility to type II hypersensitivity reaction(MP:0005612)

  • decreased trabecular bone mass(MP:0010873)

  • hematopoietic system phenotype(MP:0005397)

  • immune system phenotype(MP:0005387)

  • increased susceptibility to bacterial infection(MP:0002412)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Em, IgH promoter, human CD40 cDNA, rabbit beta-globin poly A, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse CD40 genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Inter. Immunol., 14, 319-329 (2002).
    DepositorHitoshi Kikutani (Osaka University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data