Strain Data Sheet

RBRC00782

Strain Information

Image
BRC No.RBRC00782
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Gipr<tm1Yse>/Rbrc
Former Common nameGIPR Knockout mice
H-2 Haplotype
ES Cell lineCGR8 [129P2/OlaHsd]
Background strainC57BL/6NCrSlc
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Yutaka Seino, Kyoto University in 1999. A neomycin cassette was inserted to replace exons 4 and 5 of GIPR gene. The mutant mice were backcrossed to C57BL/6.
Strain descriptionB6.129P2-Gipr<tm1Yse>. Gastric inhibitory polypeptide (GIP) is a gastrointestinal peptide hormone synthesized by K cells in the duodenum and small intestine, GIP plays a role in gastric secretory inhibition, gastric mobility, and insulin release by binding to its specific receptor, the GIP receptor (GIPR). Homozygous mutant mice for Gipr<tm1Yse> targeted mutation are fertile and viable, but exhibit hyperglycemia after high-fat diet ingestion. This strain is useful for elucidating of the signaling mechanism of GIP from pancreatic beta cell to intestine.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)Homozygous mice are fertile and viable.
References
Glucose intolerance caused by a defect in the entero-insular axis: a study in gastric inhibitory polypeptide receptor knockout mice.
Miyawaki K, Yamada Y, Yano H, Niwa H, Ban N, Ihara Y, Kubota A, Fujimoto S, Kajikawa M, Kuroe A, Tsuda K, Hashimoto H, Yamashita T, Jomori T, Tashiro F, Miyazaki J, Seino Y
Proc. Natl. Acad. Sci. USA, 96, 14843-14847 (1999). 10611300

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gipr
MGI:1352753		gastric inhibitory polypeptide receptor7Gipr<tm1Yse>
MGI:2149964		targeted mutation 1, Yutaka Seino
neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal glucose homeostasis(MP:0002078)

  • decreased circulating insulin level(MP:0002727)

  • hyperglycemia(MP:0001559)

  • impaired glucose tolerance(MP:0005293)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse GIP receptor genomic DNA, E. coli neo, mouse phosphoglycerate kinase promoter (PGK promoter)
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA, 96, 14843-14847 (1999).
    DepositorYutaka Seino (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Iida A, Seino Y, Fukami A, Maekawa R, Yabe D, Shimizu S, Kinoshita K, Takagi Y, Izumoto T, Ogata H, Ishikawa K, Ozaki N, Tsunekawa S, Hamada Y, Oiso Y, Arima H, Hayashi Y.
    Endogenous GIP ameliorates impairment of insulin secretion in proglucagon-deficient mice under moderate beta cell damage induced by streptozotocin.
    Diabetologia 59(7) 1533-1541(2016) 27053237