Strain Information | |
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Image | |
BRC No. | RBRC00850 |
Type | Targeted Mutation Congenic![]() |
Species | Mus musculus |
Strain name | B6.129P2-Itgal<tm1Mak> |
Former Common name | C57BL/6-[KO]LFA-1; B6.129P2-Itgb2<tm1Oci> |
H-2 Haplotype | |
ES Cell line | E14K [129P2/OlaHsd] |
Background strain | |
Appearance | black |
Strain development | From Tak. W. Mak of ontario Cancer Institute, N6 |
Strain description | Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice. |
Colony maintenance | |
References | LFA-1-deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor. Schmits R, Kundig T M, Baker D M, Shumaker G, Simard J J, Duncan G, Wakeham A, Shahinian A, van der Heiden A, Bachmann M F, Ohashi P S, Mak T W, Hickstein D D J. Exp. Med., 183, 1415-1426 (1996). 8666900 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |||||||
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Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
Itgal MGI:96606 | integrin alpha L | 7 | Itgal<tm1Mak> MGI:2181378 | targeted mutation 1, Tak W Mak | |||
neo | neomycin resistance gene (E. coli) | 7 | herpes simplex virus thymidine kinase promoter (HSV tk promoter) |
Phenotype | |
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Annotation by Mammalian phenotyhpe ontology | more 1 phenotypes |
Detailed phenotype data |
Ordering Information | |
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Donor DNA | herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, tk poly A, mouse CD11a genomic DNA |
Research application | Immunology and Inflammation Research Virology Research covid19_infection |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER (Tak W. Mak, Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca). |
Depositor | Shigeo Koyasu (Keio University) |
Strain Status | ![]() |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K. TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells. Nat Commun 8(1) 1592(2017) 29150602 |