Strain Data Sheet

RBRC00857

Strain Information

Image
BRC No.RBRC00857
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Treh<tm1Tkam>/Rbrc
Former Common nametrehalase Knockout mice
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strainC57BL/6JJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Masashi Kurimoto, Biochemical Laboratories, Inc. in 2001-2002. A neomycin selection cassette replaced exons 5-10 of the Treh gene.
Strain descriptionB6;B6CB-Treh<tm1Tkam>. Targeted disruption of the trehalase gene. The mice with this mutant allele lack the trehalase activity in small intestine and kidney.
Colony maintenanceSibling x Sibling (Homozygote x Homozygote)Homozygous mutant mice are viable and fertile.
References
Nutr. Res., 23, 287-298 (2003).

Trehalose itself plays a critical role on lipid metabolism: Trehalose increases jejunum cytoplasmic lipid droplets which negatively correlated with mesenteric adipocyte size in both HFD-fed trehalase KO and WT mice.
Chikako Arai, Aki Suyama, Shigeyuki Arai, Norie Arai, Chiyo Yoshizane, Satomi Koya-Miyata, Akiko Mizote, Shin Endo, Toshio Ariyasu, Hitoshi Mitsuzumi, Shimpei Ushio
Nutr. Metab. (Lond), 17:22 (2020). 32206077

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Treh
MGI:1926230		trehalase (brush-border membrane glycoprotein)9Treh<tm1Tkam>
MGI:3586558		targeted mutation 1, Takashi Kamiya
  • diarrhea-vomiting due to trehalase deficiency(MedGEN)
    • neo neomycin resistance gene (E. coli)9 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal glucose homeostasis(MP:0002078)

  • abnormal intestinal absorption(MP:0001666)

  • digestive/alimentary phenotype(MP:0005381)

  • renal/urinary system phenotype(MP:0005367)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse trehalase genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nutr. Res., 23, 287-298 (2003). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form (Contact info: Shin Endo <shin.endo@hb.nagase.co.jp>, Hayashibara Co., Ltd.). The RECIPIENT is permitted to use the BIOLOGICAL RESOURCE only for the purpose of not-for-profit academic research. Any patent application relating to the results of the research conducted by using the BIOLOGICAL RESOURCE hereunder may not be filed without prior written consent of the DEPOSITOR. The RECIPIENT promises not to make any claim or other actions to restrict the using of the BIOLOGICAL RESOURCE by the DEPOSITOR, RIKEN BRC and any other parties receiving it from the DEPOSITOR or RIKEN BRC. The DEPOSITOR shall not be liable for RECIPIENT's performance and nonperformance in relation to the BIOLOGICAL RESOURCE between RECIPIENT and RIKEN BRC.
    DepositorMasashi Kurimoto (Hayashibara Biochemical Laboratories, Inc.)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data