Strain Data Sheet

RBRC00858

Strain Information

Image
BRC No.RBRC00858
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S6-Bcl2l12/Irf3<tm1Ttg>/TtgRbrc
Former Common nameIRF-3 KO/B6; IRF-3 Knockout mice, C57BL/6J
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strainC57BL/6JJcl
Appearanceblack
Strain developmentDeveloped by Tadatsugu Taniguchi, Graduate School of Medicine and Faculty of Medicine, University of Tokyo. C57BL/6 semicongenic strain.
Strain descriptionThe Irf3 deficient mice were created by replacing a 2.2 kb region of Irf3 containing a putative transcription initiation site and the coding N-terminal region of the DNA binding domain. The analysis of MEFs demonstrated loss of Irf3 protein. Homozygous null mice are more susceptible to viral infection such as encephalomyocarditis virus and exhibit lower serum interferon levels in response to viral infection, indicating incapability of the mutant to respond against viral infection.
Colony maintenanceHomozygote x Homozygote
References
Distinct and essential roles of transcription factors IRF-3 and IRF-7 in response to viruses for IFN-alpha/beta gene induction.
Sato M, Suemori H, Hata N, Asagiri M, Ogasawara K, Nakao K, Nakaya T, Katsuki M, Noguchi S, Tanaka N, Taniguchi T
Immunity, 13, 539-548 (2000). 11070172

Health Report

Examination Date / Room / Rack2023/08/28Room:4-CRack:ASentinel mouse program
2023/05/29Room:4-CRack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Bcl2l12
MGI:1922986		BCL2 like 127Bcl2l12<tm1Ttg>
MGI:4353426		targeted mutation 1, Tadatsugu Taniguchi
Irf3
MGI:1859179		interferon regulatory factor 37Irf3<tm1Ttg>
MGI:3577601		targeted mutation 1, Tadatsugu Taniguchi
  • herpes simplex encephalitis, susceptibility to, 7(MedGEN)
    • neo neomycin resistance gene (E. coli)7 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal circulating interferon level(MP:0008548)

  • decreased cellular sensitivity to ultraviolet irradiation(MP:0008411)

  • increased susceptibility to viral infection(MP:0002418)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse IRF-3 genomic DNA, mouse Bcl2l12 genomic DNA
    Research applicationImmunology and Inflammation Research
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT shall inform the DEPOSITOR about the purpose of the use of the BIOLOGICAL RESOURCE and shall limit the use within the scope of the purpose described in this Agreement. The RECIPIENT shall inform the DEPOSITOR about the results obtained by the use of the BIOLOGICAL RESOURCE.
    DepositorTadatsugu Taniguchi (The University of Tokyo)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month May 2005
    Genetic Background

    BRC mice in Publications

    Ambite I, Wan MLY, Tran HT, Nazari A, Chaudhuri A, Krintel C, Gomes I, Sabari S, Ahmadi S, Carneiro ANBM, Ishac R, Haq F, Svanborg C.
    Multitarget mechanism of MYC inhibition by the bacterial lon protease in disease.
    Sci Rep 15(1) 6778(2025) 40000737
    Ines Ambite, Thi Hien Tran, Daniel S. C. Butler, Michele Cavalera, Murphy Lam Yim Wan, Shahram Ahmadi, Catharina Svanborg
    Therapeutic Effects of IL-1RA against Acute Bacterial Infections, including Antibiotic-Resistant Strains
    Pathogens 13 42(2023) 38251349
    Foss MH, Stevens SL, Jin H, Allen EM, Nelson D, DeFilippis V, Nilsen A, Stenzel-Poore MP.
    Identification and Characterization of Small-Molecule IRF3-Dependent Immune Activators for Pharmaceutical Development.
    ACS Chem Biol 17(5) 1073-1081(2022) 35471821
    Sin WX, Yeong JP, Lim TJF, Su IH, Connolly JE, Chin KC.
    IRF-7 Mediates Type I IFN Responses in Endotoxin-Challenged Mice.
    Front Immunol 11 640(2020) 32373120
    Liu Y, Mo CF, Luo XY, Li H, Guo HJ, Sun H, Hu S, Li LM, Wang YT, Yang SX, Chang S, Zou Q.
    Activation of Toll-Like Receptor 3 Induces Interleukin-1 Receptor Antagonist Expression by Activating the Interferon Regulatory Factor 3.
    J Innate Immun 12(4) 304-320(2020) 31865314
    Benmerzoug S, Bounab B, Rose S, Gosset D, Biet F, Cochard T, Xavier A, Rouxel N, Fauconnier L, Horsnell WGC, Ryffel B, Togbe D, Quesniaux VFJ.
    Sterile Lung Inflammation Induced by Silica Exacerbates Mycobacterium tuberculosis Infection via STING-Dependent Type 2 Immunity.
    Cell Rep 27(9) 2649-2664.e5(2019) 31141689
    Cheng WY, He XB, Jia HJ, Chen GH, Jin QW, Long ZL, Jing ZZ.
    The cGas-Sting Signaling Pathway Is Required for the Innate Immune Response Against Ectromelia Virus.
    Front Immunol 9 1297(2018) 29963044
    Ishii T, Hosoki K, Nikura Y, Yamashita N, Nagase T, Yamashita N.
    IFN Regulatory Factor 3 Potentiates Emphysematous Aggravation by Lipopolysaccharide.
    J Immunol 198(9) 3637-3649(2017) 28363903
    Zhang SM, Zhu LH, Li ZZ, Wang PX, Chen HZ, Guan HJ, Jiang DS, Chen K, Zhang XF, Tian S, Yang D, Zhang XD, Li H.
    Interferon regulatory factor 3 protects against adverse neo-intima formation.
    Cardiovasc Res 102(3) 469-79(2014) 24596398
    Loi P, Yuan Q, Torres D, Delbauve S, Laute MA, Lalmand MC, Pétein M, Goriely S, Goldman M, Flamand V.
    Interferon regulatory factor 3 deficiency leads to interleukin-17-mediated liver ischemia-reperfusion injury.
    Hepatology 57(1) 351-61(2013) 22911673
    Ysebrant de Lendonck L, Tonon S, Nguyen M, Vandevenne P, Welsby I, Martinet V, Molle C, Charbonnier LM, Leo O, Goriely S.
    Interferon regulatory factor 3 controls interleukin-17 expression in CD8 T lymphocytes.
    Proc Natl Acad Sci U S A 110(34) E3189-97(2013) 23918362
    Christensen JE, Fenger C, Issazadeh-Navikas S, Krug A, Liljestrøm P, Goriely S, Paludan SR, Finsen B, Christensen JP, Thomsen AR.
    Differential impact of interferon regulatory factor 7 in initiation of the type I interferon response in the lymphocytic choriomeningitis virus-infected central nervous system versus the periphery.
    J Virol 86(13) 7384-92(2012) 22514347
    Smith H, Liu XY, Dai L, Goh ET, Chan AT, Xi J, Seh CC, Qureshi IA, Lescar J, Ruedl C, Gourlay R, Morton S, Hough J, McIver EG, Cohen P, Cheung PC.
    The role of TBK1 and IKKε in the expression and activation of Pellino 1.
    Biochem J 434(3) 537-48(2011) 21204785
    Prantner D, Sikes JD, Hennings L, Savenka AV, Basnakian AG, Nagarajan UM.
    Interferon regulatory transcription factor 3 protects mice from uterine horn pathology during Chlamydia muridarum genital infection.
    Infect Immun 79(10) 3922-33(2011) 21788382
    Stevens SL, Leung PY, Vartanian KB, Gopalan B, Yang T, Simon RP, Stenzel-Poore MP.
    Multiple preconditioning paradigms converge on interferon regulatory factor-dependent signaling to promote tolerance to ischemic brain injury.
    J Neurosci 31(23) 8456-63(2011) 21653850
    Johnson J, Molle C, Aksoy E, Goldman M, Goriely S, Willems F.
    A conventional protein kinase C inhibitor targeting IRF-3-dependent genes differentially regulates IL-12 family members.
    Mol Immunol 48(12-13) 1484-93(2011) 21550664
    Marichal T, Bedoret D, Mesnil C, Pichavant M, Goriely S, Trottein F, Cataldo D, Goldman M, Lekeux P, Bureau F, Desmet CJ.
    Interferon response factor 3 is essential for house dust mite-induced airway allergy.
    J Allergy Clin Immunol 126(4) 836-844.e13(2010) 20673978
    Prantner D, Darville T, Nagarajan UM.
    Stimulator of IFN gene is critical for induction of IFN-beta during Chlamydia muridarum infection.
    J Immunol 184(5) 2551-60(2010) 20107183
    Molle C, Goldman M, Goriely S.
    Critical role of the IFN-stimulated gene factor 3 complex in TLR-mediated IL-27p28 gene expression revealing a two-step activation process.
    J Immunol 184(4) 1784-92(2010) 20083668
    Zhou S, Cerny AM, Zacharia A, Fitzgerald KA, Kurt-Jones EA, Finberg RW.
    Induction and inhibition of type I interferon responses by distinct components of lymphocytic choriomeningitis virus.
    J Virol 84(18) 9452-62(2010) 20592086
    Warzée B, Mesnil C, Hober D, Caloone D, Pirottin D, Garigliany MM, Desmecht D, Drion PV, Journot L, Lekeux P, Bureau F, Desmet CJ.
    Viral induction of Zac1b through TLR3- and IRF3-dependent pathways.
    Mol Immunol 48(1-3) 119-27(2010) 20947170
    Marsh B, Stevens SL, Packard AE, Gopalan B, Hunter B, Leung PY, Harrington CA, Stenzel-Poore MP.
    Systemic lipopolysaccharide protects the brain from ischemic injury by reprogramming the response of the brain to stroke: a critical role for IRF3.
    J Neurosci 29(31) 9839-49(2009) 19657036
    Abe T, Kaname Y, Wen X, Tani H, Moriishi K, Uematsu S, Takeuchi O, Ishii KJ, Kawai T, Akira S, Matsuura Y.
    Baculovirus induces type I interferon production through toll-like receptor-dependent and -independent pathways in a cell-type-specific manner.
    J Virol 83(15) 7629-40(2009) 19474102
    Dogusan Z, García M, Flamez D, Alexopoulou L, Goldman M, Gysemans C, Mathieu C, Libert C, Eizirik DL, Rasschaert J.
    Double-stranded RNA induces pancreatic beta-cell apoptosis by activation of the toll-like receptor 3 and interferon regulatory factor 3 pathways.
    Diabetes 57(5) 1236-45(2008) 18223009
    Sakaguchi T, Kato A, Kiyotani K, Yoshida T, Nagai Y.
    Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus-mouse system.
    Proc Jpn Acad Ser B Phys Biol Sci 84(10) 439-51(2008) 19075516
    Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S.
    IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
    J Immunol 178(12) 7607-15(2007) 17548596
    Kiyotani K, Sakaguchi T, Kato A, Nagai Y, Yoshida T.
    Paramyxovirus Sendai virus V protein counteracts innate virus clearance through IRF-3 activation, but not via interferon, in mice.
    Virology 359(1) 82-91(2007) 17027894
    Goriely S, Molle C, Nguyen M, Albarani V, Haddou NO, Lin R, De Wit D, Flamand V, Willems F, Goldman M.
    Interferon regulatory factor 3 is involved in Toll-like receptor 4 (TLR4)- and TLR3-induced IL-12p35 gene activation.
    Blood 107(3) 1078-84(2006) 16219795