Strain Data Sheet

RBRC00891

Strain Information

Image
BRC No.RBRC00891
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Spen<tm1Hon>/HonRbrc
Former Common nameMINT-KO (C57BL/6 background)
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Dr. Tasuku Honjo, Kyoto University in 2003. A pgk-neo was transfered into E14 ES cells to replace nucleotide 4891 of the coding region of Mint. The Mint deficient mice were backcrossed to C57BL/6 for 16 times.
Strain descriptionB6.129P2-Mint-pending<tm1Hon>/HonRbrc. Mint, Msx2 interacting nuclear target protein which is a suppressor factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Homozygous mutant mice are embryonic lethal due to developmental abnormality of heart, spleen and liver. This strain is useful for elucidating of Notch/RBP-J signaling pathway mechanism in mammals.
Colony maintenanceBackcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)The reproductive performance is good. Homozygous mutant mice are embryonic lethal.
References
Regulation of marginal zone B cell development by MINT, a suppressor of Notch/RBP-J signaling pathway.
Kuroda K, Han H, Tani S, Tanigaki K, Tun T, Furukawa T, Taniguchi Y, Kurooka H, Hamada Y, Toyokuni S, Honjo T
Immunity, 18, 301-312 (2003). 12594956

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Spen
MGI:1891706		spen family transcription repressor4Spen<tm1Hon>
MGI:2667509		targeted mutation 1, Tasuku Honjo
neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal B cell differentiation(MP:0002144)

  • abnormal heart development(MP:0000267)

  • abnormal pancreas development(MP:0003934)

  • lethality throughout fetal growth and development, complete penetrance(MP:0011099)

  • liver hypoplasia(MP:0000600)
    • more 2 phenotypes
  • small liver(MP:0000601)

  • translucent skin(MP:0001201)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse MINT genomic DNA, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo
    Research applicationDevelopmental Biology Research
    Immunology and Inflammation Research
    Specific Term and Conditions1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
    2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Immunity 18, 301-312 (2003).
    3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
    DepositorTasuku Honjo (Kyoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)
    Honjo Lab HP
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data