Strain Data Sheet
RBRC00906
Strain Information | |
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| Image |  |
| BRC No. | RBRC00906 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6-Tg(pLck-EGFP)1Naka/Rbrc |
| Former Common name | plck-GFP Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | C57BL/6JJcl |
| Appearance | black [(a/a B/B C/C)] |
| Strain development | Developed by Dr. Toshinori Nakayama, Graduate School of Medicine, Chiba University in 2001. The 4.2 kb pLck-EGFP plasmid was purified and injected into the pronucleous of C57BL/6J fertilized eggs. Transgenic mice were backcrossed to C57BL/6 for 4 times. |
| Strain description | C57BL/6-Tg(pLck-EGFP)1Naka. The plck-GFP transgenic mice carry EGFP (enhanced green fluorescence protein) cDNA under control of the proximal promoter of Lck (lymphocyte protein tryosine kinase). The Lck proximal promoter is reported to be most active in immature thymocytes. These transgenic mice are powerful tools for the visualization of the intracellular machinery that is crucial for the development of lymphocytes. |
| Colony maintenance | Backcross to C57BL/6J (Hemizygote x C57BL/6JJcl).Reproductive performance is good. |
| References | Progression of T cell lineage restriction in the earliest subpopulation of murine adult thymus visualized by the expression of lck proximal promoter activity. Shimizu C, Kawamoto H, Yamashita M, Kimura M, Kondou E, Kaneko Y, Okada S, Tokuhisa T, Yokoyama M, Taniguchi M, Katsura Y, Nakayama T Int. Immunol., 13, 105-117 (2001). 11133839 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| GFP | Green Fluorescent Protein (Aequorea victoria) | UN | lck proximal promoter, human growth hormone enhancer | ||||
| GH | Growth hormone polyA (human) | UN | GH | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | proximal lck promoter, Jellyfish GFP cDNA, human GH enhancer |
| Research application | Fluorescent Proteins/lacZ System Immunology and Inflammation Research |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int. Immunol., 13(1) 105-117 (2001). The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Toshinori Nakayama (Chiba University) |
| Strain Status |  Frozen embryos |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |