Strain Data Sheet

RBRC00961

Strain Information

Image
BRC No.RBRC00961
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gal3st1<tm1Kho>/KhoRbrc
Former Common nameC57BL/6N x CST KO-1(hetero), TgH(CSTneo) 01
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NJcl
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Dr. Koichi Honke, Osaka University Medical School in 1999. A targeting vector including pgk-neo was transfected into E14.1 ES cells to disrupt exons 2 and 3 of Cst gene. The Cst knockout mice were backcrossed to C57BL/6N for 6 times. Generation: N5+N1F3
Strain descriptionB6;129P2-Gal3st1<tm1Kho>. Cerebroside sulfotransferase (Cst) gene is located in the Golgi membrane that is responsible for the biosynthesis of two sulfated glycolipids (GalCer and GalEAG) in the mammals. Cst null mice lack sulfatide in myelin and sulfated seminolipid in sperm. Homozygous mutant males are sterile. This strain is useful for elucidation of the role of sulfoglycolipids in myelin function and spermatogenesis.
Colony maintenance
References
A myelin galactolipid, sulfatide, is essential for maintenance of ion channels on myelinated axon but not essential for initial cluster formation.
Ishibashi T, Dupree J L, Ikenaka K, Hirahara Y, Honke K, Peles E, Popko B, Suzuki K, Nishino H, Baba H
J. Neurosci., 22, 6507-6514 (2002). 12151530

Paranodal junction formation and spermatogenesis require sulfoglycolipids.
Honke K, Hirahara Y, Dupree J, Suzuki K, Popko B, Fukushima K, Fukushima J, Nagasawa T, Yoshida N, Wada Y, Taniguchi N
Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). 11917099

Sulfatide is a negative regulator of oligodendrocyte differentiation: development in sulfatide-null mice.
Hirahara Y, Bansal R, Honke K, Ikenaka K, Wada Y
Glia, 45, 269-277 (2004). 14730700

Cerebroside sulfotransferase deficiency ameliorates L-selectin-dependent monocyte infiltration in the kidney after ureteral obstruction.
Ogawa D, Shikata K, Honke K, Sato S, Matsuda M, Nagase R, Tone A, Okada S, Usui H, Wada J, Miyasaka M, Kawashima H, Suzuki Y, Suzuki T, Taniguchi N, Hirahara Y, Tadano-Aritomi K, Ishizuka I, Tedder T F, Makino H
J. Biol. Chem., 279, 2085-2090 (2004). 14583626

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gal3st1
MGI:1858277		galactose-3-O-sulfotransferase 111Gal3st1<tm1Kho>
MGI:2386400		targeted mutation 1, Koichi Honke
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal axon morphology(MP:0005404)

  • abnormal brain morphology(MP:0002152)

  • abnormal involuntary movement(MP:0003492)

  • abnormal myelination(MP:0000920)

  • abnormal paranode morphology(MP:0010734)
    • more 11 phenotypes
  • abnormal spermatogonia proliferation(MP:0002685)

  • abnormal testis morphology(MP:0001146)

  • arrest of male meiosis(MP:0008261)

  • ataxia(MP:0001393)

  • azoospermia(MP:0005159)

  • decreased testis weight(MP:0004852)

  • impaired limb coordination(MP:0001524)

  • limp posture(MP:0001506)

  • male infertility(MP:0001925)

  • tremors(MP:0000745)

  • weakness(MP:0000746)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Gal3st1 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002).
    DepositorKoichi Honke (Kochi University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Zhang Y, Hayashi Y, Cheng X, Watanabe T, Wang X, Taniguchi N, Honke K.
    Testis-specific sulfoglycolipid, seminolipid, is essential for germ cell function in spermatogenesis.
    Glycobiology 15(6) 649-54(2005) 15659616