Strain Data Sheet

RBRC00962

Strain Information

Image
BRC No.RBRC00962
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gal3st1<tm2Kho>/KhoRbrc
Former Common nameC57BL/6N x CST KO-2(hetero), TgH(CSTneo) 02
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strainC57BL/6NJcl
Appearance
Strain development
Strain description
Colony maintenance
References
Paranodal junction formation and spermatogenesis require sulfoglycolipids.
Honke K, Hirahara Y, Dupree J, Suzuki K, Popko B, Fukushima K, Fukushima J, Nagasawa T, Yoshida N, Wada Y, Taniguchi N
Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002). 11917099

A myelin galactolipid, sulfatide, is essential for maintenance of ion channels on myelinated axon but not essential for initial cluster formation.
Ishibashi T, Dupree J L, Ikenaka K, Hirahara Y, Honke K, Peles E, Popko B, Suzuki K, Nishino H, Baba H
J. Neurosci., 22, 6507-6514 (2002). 12151530

Sulfatide is a negative regulator of oligodendrocyte differentiation: development in sulfatide-null mice.
Hirahara Y, Bansal R, Honke K, Ikenaka K, Wada Y
Glia, 45, 269-277 (2004). 14730700

Cerebroside sulfotransferase deficiency ameliorates L-selectin-dependent monocyte infiltration in the kidney after ureteral obstruction.
Ogawa D, Shikata K, Honke K, Sato S, Matsuda M, Nagase R, Tone A, Okada S, Usui H, Wada J, Miyasaka M, Kawashima H, Suzuki Y, Suzuki T, Taniguchi N, Hirahara Y, Tadano-Aritomi K, Ishizuka I, Tedder T F, Makino H
J. Biol. Chem., 279, 2085-2090 (2004). 14583626

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gal3st1
MGI:1858277		galactose-3-O-sulfotransferase 111Gal3st1<tm1Kho>
MGI:2386400		targeted mutation 1, Koichi Honke
neo neomycin resistance gene (E. coli)11 mouse phosphoglycerate kinase promoter (PGK promoter)

Phenotype

Annotation by Mammalian phenotyhpe ontology
  • abnormal axon morphology(MP:0005404)

  • abnormal brain morphology(MP:0002152)

  • abnormal involuntary movement(MP:0003492)

  • abnormal myelination(MP:0000920)

  • abnormal paranode morphology(MP:0010734)
    • more 11 phenotypes
  • abnormal spermatogonia proliferation(MP:0002685)

  • abnormal testis morphology(MP:0001146)

  • arrest of male meiosis(MP:0008261)

  • ataxia(MP:0001393)

  • azoospermia(MP:0005159)

  • decreased testis weight(MP:0004852)

  • impaired limb coordination(MP:0001524)

  • limp posture(MP:0001506)

  • male infertility(MP:0001925)

  • tremors(MP:0000745)

  • weakness(MP:0000746)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Gal3st1 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 99, 4227-4232 (2002).
    DepositorKoichi Honke (Kochi University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data