Strain Data Sheet

RBRC00983

Strain Information

Image
BRC No.RBRC00983
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Gata2<tm1Mym>/Rbrc
Former Common nameGATA-2 2nd Exon GFP knock in
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6J
Appearanceblack [a/a B/B C/C]
Strain developmentDeveloped by Drs. Naoko Minegishi and Masayuki Yamamoto, Center for Tukuba Advanced Research Alliance, University of Tsukuba in 2003. GFP was inserted in exon 2 of the Gata2 gene (Blood, 2003).
Strain descriptionB6;129P2-Gata2<tm1Mym>. GFP knock-in mice in the Gata2 locus, replacing the endogenous Gata2 coding sequences in the second exon. GFP expression was observed in the early hematopoietic tissues. Homozygous mutat mice are embryonic lethal (E11.0).
Colony maintenanceHeterozygote x Wild-type [C57BL/6J]Homozygous mutat mice are embryonic lethal (E11.0).
References
Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos.
Minegishi N, Suzuki N, Yokomizo T, Pan X, Fujimoto T, Takahashi S, Hara T, Miyajima A, Nishikawa S, Yamamoto M
Blood, 102, 896-905 (2003). 12689939

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
GFP Green Fluorescent Protein (Aequorea victoria)6
Gata2
MGI:95662		GATA binding protein 26Gata2<tm1Mym>
MGI:2673268		targeted mutation 1, Masayuki Yamamoto
  • acute myeloid leukemia(DisGeNET, MedGEN)

  • deafness-lymphedema-leukemia syndrome(MedGEN)

  • monocytopenia with susceptibility to infections(DisGeNET, MedGEN)
    • more 1 Diseases
  • myelodysplastic syndrome(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
  • abnormal blood cell morphology/development(MP:0002429)

  • abnormal definitive hematopoiesis(MP:0002123)

  • embryonic lethality during organogenesis, complete penetrance(MP:0011098)
    • Detailed phenotype data

    Ordering Information

    Donor DNAJellyfish GFP cDNA, mouse GATA-2 genomic DNA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The depositor should be a co-author in articles when the users publish their data using these mice for 2 year after provided. Users should contact the depositor in the case of application for any patents with the results from these mice.
    DepositorMasayuki Yamamoto (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP (Japanese)
    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data